TP53 Recombinant Antibody AE00124

520,00

Selective and specific rabbit recombinant antibody clone TP53/2092R to human TP53

See all our TP53 antibodies

Official Symbol: TP53

GeneID: 7157

Swiss-Prot: PP04637

Description

TP53

All names and symbols: Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor protein p53; Tumor suppressor p53; BCC7; LFS1; P53; TRP53

Host speciesRabbit
ClonalityRecombinant
IsotypeIgG
Confirmed species reactivityHuman
Confirmed applicationsIHC, PA, WB
Aeonian Rating®100
RRIDAB_2736890

Immunohistochemistry (IHC):

AE00124 to TP53 was successfully used to stain human colon carcinoma sections. Recommended concentration: 0.3-1ug/ml

IHC of human colon carcinoma stained with TP53 Rabbit Recombinant Monoclonal Antibody AE00124
Human colon carcinoma with AE00124

Formaldehyde-fixed, paraffin-embedded human colon carcinoma stained with TP53 Recombinant Rabbit Antibody AE00124 at 0.5-1ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

Western Blot (WB):

AE00124 to TP53 was successfully used to stain an approx. 53kDa band in lysates of cell line HeLa. Recommended concentration: 0.3-1ug/ml

WB of HeLa stained with TP53 Rabbit Recombinant Monoclonal Antibody AE00124
HeLa with AE00124

Western Blot of a HeLa lysate (30ug) stained with TP53 Rabbit Recombinant Antibody AE00124 at 0.5-1ug/ml (1h at ambient temp). ECL staining by HRP.

Evidence of mono-specificity (in native PA):

Specificity and selectivity of AE00124 to TP53 were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed the following closely related protein: TP73 and TP63. These proteins were part of the array used and showed no cross-reactivity signals.

AE00124 on protein array
AE00124 on HuProt(TM) array

Cross-reactivity assessment of TP53 Rabbit Recombinant Antibody AE00124 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified TP53 Rabbit Recombinant Antibody AE00124. Confirmation of Purity and Integrity of Antibody.

Coomassie-stained AE00124
AE00124 Coomassie bands

Integrity of the purified antibody AE00124 under non-reduced and reduced conditions, showing intact IgG at around 100kDa (NR) and intact heavy and light chains at 50kDa and 25kDa resp. (R)

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant full length human TP53 protein

Epitope

aa 16-25

Cellular localization

ER, mitochondia, nucleus

Positive Controls

breast carcinoma, colon carcinoma, MDA-MB-231

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