Cat nr AE00124 Recombinant Rabbit Monoclonal Antibody, clone TP53/2092R to:
TP53, Tumor protein p53
BCC7, LFS1, P53, TRP53, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

Cellular localization
Nucleus, some isoforms may end up in ER and mitochondrion
Official Symbol (Gene)
TP53
GeneID
7157
Swiss-Prot
Applications
IHC, WB
Positive controls
MDA-MB-231, breast or colon carcinoma.
Aeonian Rating®
100
Positive controls
Isotype Rabbit IgG
Species reactivity Human
Immunogen Recombinant human wild-type p53 protein
Epitope aa 16-25
Purification By Protein A from bioreactor concentrate
Formulations 200ug IgG/ml in PBS, 0.05% BSA, 0.05% azide (20ug or 100ug)
1mg IgG/ml in PBS (100ug, or contact us)
Storage instructions Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20C or -80C and keep one aliquot at 4C for daily experimentations. Azide will preserve antibody at 4C for 6-12 months, when kept away from direct sun light.
Expiration Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Warranty This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
Liability This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.

Immunohistochemistry (IHC):

AE00124 to TP53 was successfully used to stain human colon carcinoma sections. Recommended concentration: 0.3-1ug/ml

Human colon carcinoma with AE00124
Formaldehyde-fixed, paraffin-embedded human colon carcinoma stained with TP53 Recombinant Rabbit Monoclonal Antibody AE00124 at 0.5-1ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

Western Blot (WB):

AE00124 to TP53 was successfully used to stain an approx. 53kDa band in lysates of cell line HeLa. Recommended concentration: 0.3-1ug/ml

HeLa with AE00124
Western Blot of a HeLa lysate (30ug) stained with TP53 Rabbit Recombinant Monoclonal Antibody AE00124 at 0.5-1ug/ml (1h at ambient temp). ECL staining by HRP.

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified TP53 Rabbit Recombinant Monoclonal Antibody AE00124. Confirmation of Purity and Integrity of Antibody.

AE00124 Coomassie bands
Integrity of the purified antibody AE00124 under non-reduced and reduced conditions, showing intact IgG at around 100kDa (NR) and intact heavy and light chains at 50kDa and 25kDa resp. (R)

Evidence of mono-specificity:

Specificity and selectivity of AE00124 to TP53 were tested on a human protein array, showing monospecificity, against >19,000 full-length human proteins. A protein BLAST search against H. sapiens reveal the following closely related protein: TP73 and TP63. These proteins were part of the array used and showed no cross-reactivity signals.

AE00124 on HuProt(TM) array
Cross-reactivity assessment of TP53 Rabbit Recombinant Monoclonal Antibody AE00124 (1ug/ml) on CDI's Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

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