B2M Recombinant Antibody AE00142

520,00

Selective and specific mouse recombinant antibody clone rB2M/961 to human B2M

Official Symbol: B2M

GeneID: 567

Swiss-Prot: P61769

Description

B2M

All names and symbols: Beta-2-microglobulin; B2M; IMD43

Host speciesMouse
ClonalityRecombinant
IsotypeIgG2b, kappa
Confirmed species reactivityHuman
Confirmed applicationsIHC, PA. WB
Aeonian Rating®90
RRIDAB_2737013

Immunohistochemistry (IHC):

AE00142 to B2M was successfully used to stain carcinoma in human lung, cervix and colon sections. Recommended concentration: 0.3-1ug/ml

IHC of human cervival carcinoma stained with B2M Mouse Recombinant Monoclonal Antibody AE00142
Human cervical carcinoma with AE00142

Formaldehyde-fixed, paraffin-embedded human cervical carcinoma stained with B2M Mouse Recombinant Antibody AE00142 at 0.5-1ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human colon carcinoma stained with B2M Mouse Recombinant Monoclonal Antibody AE00142
Human colon carcinoma with AE00142

Formaldehyde-fixed, paraffin-embedded human colon carcinoma stained with B2M Mouse Recombinant Antibody AE00142 at 0.5-1ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human lung carcinoma stained with B2M Mouse Recombinant Monoclonal Antibody AE00142
Human lung carcinoma with AE00142

Formaldehyde-fixed, paraffin-embedded human lung carcinoma stained with B2M Mouse Recombinant Antibody AE00142 at 0.5-1ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

Western Blot (WB):

AE00142 to B2M was successfully used to stain an approx. 12kDa band in kidney lysates. Recommended concentration: 0.3-1ug/ml

WB of human kidney stained with B2M Mouse Monoclonal Antibody AE00142
Human kidney with AE00142

Western Blot of a human kidney lysate (30ug) stained with B2M Mouse Recombinant Antibody AE00142 at 0.5-1ug/ml (1h at ambient temp). ECL staining by HRP.

Evidence of mono-specificity (in native PA):

Specificity and selectivity of AE00142 to B2M were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed no closely related other proteins.

AE00142 on protein array
AE00142 on HuProt(TM) array

Cross-reactivity assessment of B2M Mouse Recombinant Antibody AE00142 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified B2M Mouse Recombinant Antibody AE00142. Confirmation of Purity and Integrity of Antibody.

Coomassie-stained AE00142
AE00142 Coomassie bands

Integrity of the purified antibody AE00142 under non-reduced and reduced conditions, showing intact IgG at around 150kDa (NR) and intact heavy and light chains at 50kDa and 25kDa resp. (R)

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant full-length human B2M protein

Epitope

Unknown

Cellular localization

secreted

Positive Controls

cervical carcinoma, colon carcinoma, endometrial carcinoma, HeLa, HL-60, lymphoma, melanoma, renal carcinoma, stomach carcinoma

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