CD79b Monoclonal Antibody AE00162

520,00

Selective and specific mouse monoclonal antibody clone IGB/1844 to human CD79b

Official Symbol: CD79B

GeneID: 974

Swiss-Prot: P40259

Description

CD79b

All names and symbols: B-cell antigen receptor complex-associated protein beta chain; B-cell-specific glycoprotein B29; CD79b molecule; Ig-beta, Immunoglobulin-associated B29 protein; CD_antigen: CD79b; AGM6; B29; IGB

Host speciesMouse
ClonalityMonoclonal
IsotypeIgG1, kappa



Confirmed species reactivityHuman
Confirmed applicationsIHC, PA
Aeonian Rating®83
RRIDAB_2750608



Immunohistochemistry (IHC):

AE00162 to CD79b was successfully used to stain germinal centre B-cells in human tonsil sections. Recommended concentration: 1-3ug/ml

IHC of human tonsil stained with CD79b Mouse Monoclonal Antibody AE00162
Human tonsil with AE00162

Formaldehyde-fixed, paraffin-embedded human tonsil stained with CD79b Mouse Monoclonal Antibody AE00162 at 1-2ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

Evidence of mono-specificity (in native PA):

Specificity and selectivity of AE00162 to CD79b were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed no closely related other proteins.

AE00162 on protein array
AE00162 on HuProt(TM) array

Cross-reactivity assessment of CD79b Mouse Monoclonal Antibody AE00162 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified CD79b Mouse Monoclonal Antibody AE00162. Confirmation of Purity and Integrity of Antibody.

Coomassie-stained AE00162
AE00162 Coomassie bands

Integrity of the purified antibody AE00162 under non-reduced and reduced conditions, showing intact IgG at around 150kDa (NR) and intact heavy and light chains at 50kDa and 25kDa resp. (R)

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein G from bioreactor concentrate

Immunogen

Recombinant fragment around aa 29-159 of human CD79b protein (exact sequence is proprietary)

Epitope

Extracellular domain (within aa 27-159 region)

Cellular localization

cell surface, plasma membrane

Positive Controls

Daudi, lymph node, Ramos, tonsil

clonality

monoclonal

host species

Mouse

Isotype

IgG1

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