EGFR-pY845 Polyclonal Antibody AE00212

520,00825,00

Phospho-specific rabbit polyclonal antibody to human EGFR, phosphorylated Y845 (EGFR-pY845)

See all our EGFR antibodies

Official Symbol: EGFR

GeneID: 1956

Swiss-Prot: P00533

Description

EGFR-pY845

All names and symbols: Epithelial Growth Factor Receptor; EGFR; ERBB; ERBB1; HER1; NISBD2; PIG61; mENA

Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Confirmed species reactivityHuman
Confirmed applicationsWB
Aeonian Rating®70
RRIDAB_2813793

Western Blot (WB):

AE00212 to EGFR-pY845 was successfully used to stain an approx. 200kDa band in lysates of A431 after overnight serum starvation and subsequent treatment with EGF. Recommended concentration: 1-3ug/ml

WB of A431 stained with EGFR-pY845 Rabbit Phospho-Specific Polyclonal Antibody AE00212
A431 with AE00212

Western Blot of 30ug lysate from serum-starved A431 with or without stimulation by 100mg/ml EGF (30 min, 37C) ), stained with EGFR-pY845 Rabbit Polyclonal Antibody AE00212 at 1ug/ml (1h at ambient temp). ECL staining by HRP.

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

Antigen-affinity purified from serum by sequential phospho-peptide and non-phospho-peptide affinity columns

Immunogen

A synthetic peptide around phosphorylated Y869 of human EGFR (NP_005219.2) corresponding to Y845 of the mature protein (starts with L25).

Epitope

A short sequence around phosphorylated Y845 (exact sequence is proprietary)

Cellular localization

cell surface, plasma membrane, translocation to nucleus upon EGF stimulation

Positive Controls

EGF-stimulated serum-starved A431

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