NPM1-pT199 Polyclonal Antibody AE00223

520,00825,00

Phospho-specific rabbit polyclonal antibody to human NPM1, phosphorylated T199 (NPM1-pT199)

See all our NPM1 antibodies

Official Symbol: NPM1

GeneID: 4869

Swiss-Prot: P06748

Description

NPM1-pT199

All names and symbols: Nucleolar phosphoprotein B23; Nucleolar protein NO38; Nucleophosmin; Nucleophosmin 1; Nucleophosmin-1; Numatrin; NPM1

Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Confirmed species reactivityHuman, Rat
Confirmed applicationsWB
Aeonian Rating®70
RRIDAB_2814719

Western Blot (WB):

AE00223 to NPM1-pT199 was successfully used to stain an approx. 39kDa band in lysates of HeLa and PC12 after Nocodazole treatment.. Recommended concentration: 1-3ug/ml

WB of HeLa and PC12 stained with NPM1-pT119 Rabbit Phospho-Specific Polyclonal Antibody AE00223
HeLa and PC12 with AE00223

Western Blots of 30ug HeLa and PC12 lysates with or without Nocodazole (100ng/ml, 24h and 1ug/ml, 16h resp.) treatment, stained with NPM1-pT199 Rabbit Polyclonal Antibody AE00223 at 1ug/ml (1h at ambient temp). ECL staining by HRP.

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

Antigen-affinity purified from serum by sequential phospho-peptide and non-phospho-peptide affinity columns

Immunogen

A synthetic peptide around phosphorylated T119 of human NPM1 (NP_001032827.1)

Epitope

A short sequence around phosphorylated T119: RDTPA

Cellular localization

nucleus

Positive Controls

Nocodazole-treated HeLa, Nocodazole-treated PC12

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