EGFR Recombinant Antibody AE00248

520,00

Selective and specific rabbit recombinant antibody clone GFR/2968R to human EGFR

See all our EGFR antibodies

Official Symbol: EGFR

GeneID: 1956

Swiss-Prot: P00533

Description

EGFR

All names and symbols: Epithelial Growth Factor Receptor; EGFR; ERBB; ERBB1; HER1; NISBD2; PIG61; mENA

Host speciesRabbit
ClonalityRecombinant
IsotypeIgG
Confirmed species reactivityHuman
Confirmed applicationsIHC, PA, WB
Aeonian Rating®90
RRIDAB_2814960

Immunohistochemistry (IHC):

AE00248 to EGFR was successfully used to stain syncytiotrophoblasts in human placenta sections. Recommended concentration: 1-3ug/ml

IHC of human placenta stained with EGFR Rabbit Recombinant Monoclonal Antibody AE00248
Human placenta with AE00248

Formaldehyde-fixed, paraffin-embedded human placenta stained with EGFR Rabbit Recombinant Antibody AE00248 at 1-2ug/ml for 30 minutes at RT. Epitope retrieval: boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

Western Blot (WB):

AE00248 to EGFR was successfully used to stain an approx. 150kDa band in lysates of cell line A431. Recommended concentration: 1-3ug/ml

WB of A431 stained with EGFR Rabbit Recombinant Monoclonal Antibody AE00248
A431 with AE00248

Western Blot of a A431 lysate (30ug) stained with EGFR Rabbit Recombinant Antibody AE00248 at 1ug/ml (1h at ambient temp). ECL staining by HRP.

Evidence of mono-specificity (in native PA):

Specificity and selectivity of AE00248 to EGFR were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed the following closely related proteins: HER2, HER3 and HER4. These proteins were part of the array used and showed no cross-reactivity signals.

AE00248 on protein array
AE00248 on HuProt(TM) array

Cross-reactivity assessment of EGFR Rabbit Recombinant Antibody AE00248 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified EGFR Rabbit Recombinant Monoclonal Antibody AE00248. Confirmation of Purity and Integrity of Antibody.

Coomassie-stained AE00248
AE00248 Coomassie bands

Integrity of the purified antibody AE00248 under non-reduced and reduced conditions, showing intact IgG at around 120kDa (NR) and intact heavy and light chains at 50kDa and 25kDa resp. (R).

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant full length EGFR protein

Epitope

Unknown

Cellular localization

cell surface, plasma membrane

Positive Controls

A431, carcinoma, placenta

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