Finding new pairs for sandwich ELISA:

To make a commercially successful kit you may need to identify the optimal pair by combining products from two different sources. This identification process can be difficult and expensive since so many antibodies occur as different brands in so many catalogues. This increases the risk of testing identical products obtained from different sources. Here is where we can help out. Your plans remain strictly confidential, when we identify the best antibody candidates straight from the original manufacturers.

Have you got your developmental team set up, then we provide you with the strongest candidates to let you identify the optimal pair for your kit. If not, we will test the antibodies to find the optimal pair for your kit (possibly with your own favourite). We make sure the paired antibodies are in sufficient supply and they do not suffer from severe batch-to-batch variations (Voskuil, 2017). The manufacturers of the paired antibodies will be revealed to you afterwards for undisruptive supply.

Antibody manufacturers who want to make products available for sandwich ELISA testing are invited to work with us. Our contacts are keen to have your products tested against those of others to find the optimal pairs. We seek purified monoclonal antibodies (preferably with known epitope and we love recombinant antibodies), and polyclonal peptide antibodies (antigen affinity-purified and with known antigen sequence). In addition we seek antigen-affinity purified polyclonal antibodies against the entire protein of interest.

Have your antibodies tested in ELISA, IHC or WB:

Any catalogue with antibodies without proper data for its most popular application can now cost-effectively outsource this work to us. We will provide the service with the highest quality standard, we will determine (when you don't) on what tissue type or cell type the antibody needs tested, and we will return the results to the customer accompanied with our scientific assessment. At this stage we offer ELISA, IHC and WB testing, and we will expand to ICC and FC.

Delivery of a high standard staining report

All results are delivered with a scientific Aeonian staining report in Excel, including all experimental conditions, objective description of the observed staining pattern, pass or fail judgement, and if passed, captions and legends ready for the product sheet. All pictures are delivered in JPG or TIFF format at high resolution.

For IHC assessments, pictures come in two magnifications and include separate results from isotype negative controls. Our contracted provider is focussed on projects for the pharma industries, and we are privileged to have access to them for assessments of research antibodies.

Thus, our service is unique and delivers a complete package taking away any concerns about data being fit or unfit for the catalogue. Please see examples from recent projects below.

Immunohistochemistry (IHC)

Human hippocampus affected by Alzheimer's disease shows Abeta-staining of amyloid plaques in the parahippocampal gyrus. All experimental conditions are delivered to the customer.

AD hippocampus Ab00714 3.3x 2
AD hippocampus Ab00714 20x 2

Anti-Amyloid beta Ab00714 (4µg/ml) staining of paraffin embedded human hippocampus affected by Alzheimer's disease. Insert: Isotype control anti-fluorescein Ab00102 (4ug/ml) at same magnification. Microwaved antigen retrieval with citrate buffer pH 6, DAB-staining by anti-rabbit-HRP secondary.

Human lung shows strong staining of type II pneumocytes. All experimental conditions are delivered to the customer.

Lung Ab00712 6.0x 2
Lung Ab00712 40x 2

Anti-MUC1 Ab00712 (4µg/ml) staining of paraffin embedded human lung. Insert: Isotype control anti-fluorescein Ab00102 (4ug/ml) at same magnification. Microwaved antigen retrieval with citrate buffer pH 6, DAB-staining by anti-rabbit-HRP secondary.

Human tonsil shows strong membrane staining of some T-cells at the periphery of the germinal centre and between follicles. All experimental conditions are delivered to the customer.

Tonsil Ab00170 13x 2
Tonsil Ab00170 40x 2

Anti-CD3 epsilon Ab00170 (4µg/ml) staining of paraffin embedded human tonsil. Insert: Isotype control anti-fluorescein Ab00102 (4ug/ml) at same magnification. Microwaved antigen retrieval with citrate buffer pH 6, DAB-staining by anti-rabbit-HRP secondary.