|Immunogen||Recombinant human wild-type p53 protein|
|Purification||By Protein A from bioreactor concentrate|
|Formulations||200ug IgG/ml in PBS, 0.05% BSA, 0.05% azide (20ug or 100ug)|
|1mg IgG/ml in PBS (100ug, or contact us)|
|Storage instructions||Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20C or -80C and keep one aliquot at 4C for daily experimentations. Azide will preserve antibody at 4C for 6-12 months, when kept away from direct sun light.|
|Expiration||Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below.|
|Warranty||This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.|
|Liability||This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.|
AE00124 to TP53 was successfully used to stain human colon carcinoma sections. Recommended concentration: 0.3-1ug/ml
Western Blot (WB):
AE00124 to TP53 was successfully used to stain an approx. 53kDa band in lysates of cell line HeLa. Recommended concentration: 0.3-1ug/ml
Evidence of molecular integrity:
SDS-PAGE Analysis of Purified TP53 Rabbit Recombinant Monoclonal Antibody AE00124. Confirmation of Purity and Integrity of Antibody.
Evidence of mono-specificity:
Specificity and selectivity of AE00124 to TP53 were tested on a human protein array, showing monospecificity, against >19,000 full-length human proteins. A protein BLAST search against H. sapiens reveal the following closely related protein: TP73 and TP63. These proteins were part of the array used and showed no cross-reactivity signals.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).