Description
ADORA2A
All names and symbols: A2A Adenosine Receptor; Adenosine A2A Receptor; Adenosine receptor A2a; A2AAR; A2aR; RDC8; ADORA2
| Host species | Mouse |
| Clonality | Recombinant |
| Isotype | IgG1, kappa, recombinant version of clone IgG2838 |
| Confirmed species reactivity | Human |
| Confirmed applications | SPR, blocking |
| Aeonian Rating® | 95 |
| RRID | AB_2904503 |
Immunoassays
Surface Plasmon Resonance (SPR):
The Fab generated from the original clone Ig2838 was successfully used in binding assays (BIAcore T100 system) onto the A2A adenosine receptor.
Hino T, Arakawa T, Iwanari H, Yurugi-Kobayashi T, Ikeda-Suno C, Nakada-Nakura Y, Kusano-Arai O, Weyand S, Shimamura T, Nomura N, Cameron AD, Kobayashi T, Hamakubo T, Iwata S, Murata T. G-protein-coupled receptor inactivation by an allosteric inverse-agonist antibody. Nature. 2012 Jan 29;482(7384):237-40. doi: 10.1038/nature10750. PMID: 22286059.
Biological Activity
Blocking the receptor:
The Fab generated from the original clone Ig2838 was successfully used to lock the A2A adenosine receptor in an inactive conformation.
Hino T, Arakawa T, Iwanari H, Yurugi-Kobayashi T, Ikeda-Suno C, Nakada-Nakura Y, Kusano-Arai O, Weyand S, Shimamura T, Nomura N, Cameron AD, Kobayashi T, Hamakubo T, Iwata S, Murata T. G-protein-coupled receptor inactivation by an allosteric inverse-agonist antibody. Nature. 2012 Jan 29;482(7384):237-40. doi: 10.1038/nature10750. PMID: 22286059.
| Expiration | Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below. |
| Storage instructions | Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light. |
| Warranty | This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund. |
| Liability | This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user. |
Reviews
There are no reviews yet.