Description
His epitope tag
Epitope tag, for the detection of recombinant proteins tagged with the HHHHH sequence.
| Host species | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG, kappa, recombinant chimeric version of mouse IgG2b clone 3D5 |
| Confirmed applications | ELISA, FC, IAP, WB |
| Aeonian Rating® | 100 |
| RRID | AB_2910188 |
Immunoassays
Enzyme-Linked ImmunoSorbent Assay (ELISA):
The 3D5 His tag antibody was successfully used to detect His-tagged citrate synthase.
Lindner P, Bauer K, Krebber A, Nieba L, Kremmer E, Krebber C, Honegger A, Klinger B, Mocikat R, Plückthun A. Specific detection of his-tagged proteins with recombinant anti-His tag scFv-phosphatase or scFv-phage fusions. Biotechniques. 1997 Jan;22(1):140-9. doi: 10.2144/97221rr01. PMID: 8994661.
Flow Cytometry (FC):
The 3D5 His tag antibody was successfully used to detect immunogen-coated EL-4 murine T lymphoblast cells.
Lindner P, Bauer K, Krebber A, Nieba L, Kremmer E, Krebber C, Honegger A, Klinger B, Mocikat R, Plückthun A. Specific detection of his-tagged proteins with recombinant anti-His tag scFv-phosphatase or scFv-phage fusions. Biotechniques. 1997 Jan;22(1):140-9. doi: 10.2144/97221rr01. PMID: 8994661.
ImmunoAffinity Purification (IAP):
The 3D5 His tag antibody was successfully used to purify His-tagged ScFv proteins as a second step after immobilized metal-ion affinity chromatography.
Müller KM, Arndt KM, Bauer K, Plückthun A. Tandem immobilized metal-ion affinity chromatography/immunoaffinity purification of His-tagged proteins–evaluation of two anti-His-tag monoclonal antibodies. Anal Biochem. 1998 May 15;259(1):54-61. doi: 10.1006/abio.1998.2606. PMID: 9606143.
Western Blot (WB):
The 3D5 His tag antibody was successfully used to detect multiple His-tagged proteins from E.coli lysates.
Lindner P, Bauer K, Krebber A, Nieba L, Kremmer E, Krebber C, Honegger A, Klinger B, Mocikat R, Plückthun A. Specific detection of his-tagged proteins with recombinant anti-His tag scFv-phosphatase or scFv-phage fusions. Biotechniques. 1997 Jan;22(1):140-9. doi: 10.2144/97221rr01. PMID: 8994661.
| Expiration | Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below. |
| Storage instructions | Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light. |
| Warranty | This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund. |
| Liability | This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user. |
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