This is how we work
Antibodies need to be accompanied with convincing data to demonstrate fitness and they need to be linked to the current batch/lot. We aim to identify antibodies either with convincing data to demonstrate specificity (showing no cross-reaction with related other proteins), or with disclosed epitope or immunising peptide sequence to rule out potential cross-reactivity to related other proteins, especially when exhaustive validation data are absent. When such products are not available, we will make a clear statement regarding this for the best antibodies we do find.
We will not recommend polyclonal antibodies without batch-specific QC data. Antibodies raised against large parts or against entire proteins need to be accompanied with exhaustive and batch-specific validation data because such characteristics will not always be reproduced in new animals. However, we do have access to custom services who provide litres of antiserum pooled from a large group of animals so to circumvent batch-to-batch variations when long-term sustainable capture antibodies are required. Then, specificity comes from pairing such capture antibody with a monospecific antibody. The monospecific antibodies we identify need to be eligible for Aeonian Rating®, that is they must be forever available with proven reproducible performance from batch to batch and in a consistent formulation (with a known quantity of purified IgG).
Our special skills come to play when assessing the scientific integrity of the QC data presented by the manufacturer. Any claim of fitness for a certain application is being scrutinised by checking and comparing the evidence with those from competing other antibodies. This is a manual exercise, and therefore we do not offer an automated browsing facility. We offer custom work instead. We will engage with the identified manufacturer to discuss their presented QC data of the current batch in comparison to previous batches and to check their stock situation before reporting back to the customer. We therefore aim to serve customers who depend on a sustainable supply of product to last their projects, to support their kits or to support their catalogue
Should I just buy and try?
There are so many antibodies to the same protein on the market these days, that it becomes increasingly difficult for researchers and assay developers to find the right one(s) among them that will be fit for their specific platforms. One must buy and try quite a few without guarantee that any of them will meet their requirements. We are here to assist finding the appropriate antibodies with the characteristics you need. You can contact us directly with your specifics, and we find you the best choice(s) of products from the market. Do no longer worry about testing tens of commercial antibodies in vain. Based on your specifics, we will identify the best antibodies from the original sources by making use of our Aeonian Rating® and we will make sure that you will have sufficient supply to last your project. Your projects will be safe with us, we will always sign Non-Disclosure Agreements with our customers. For further details, please check our About Us page.
Finding new pairs for sandwich ELISA
To make a commercially successful kit you may need to identify the optimal pair by combining products from two different sources. This identification process can be difficult and expensive since so many antibodies occur as different brands in so many catalogues. This increases the risk of testing identical products obtained from different sources. Here is where we can help out. Your plans remain strictly confidential, when we identify the best antibody candidates straight from the original manufacturers.
We provide you with the strongest candidates to let you identify the optimal pair for your newly developed kit. We make sure that the selected antibodies are in sufficient supply and they do not suffer from severe batch-to-batch variations (Voskuil, 2017).