CD79a (aa202-216)
All names and symbols: IGA, MB1, MB-1, B-cell antigen receptor complex-associated protein alpha chain, Ig-alpha, MB-1 membrane glycoprotein, Membrane-bound immunoglobulin-associated protein, Surface IgM-associated protein, CD_antigen: CD79a
| Host species | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Confirmed species reactivity | Human |
| Confirmed applications | ICC, IHC, WB |
| Aeonian Rating® | 100 |
Immunocytochemistry (ICC):
AE00109 to CD79a was successfully used to stain vesicles in cell line Raji. Recommended concentration: 1-3ug/ml.
Confocal microscopy of PFA-fixed Raji stained with CD79a Rabbit Recombinant Antibody AE00109 at 1-2ug/ml (1h at ambient temp). Detection by CF488 (green) for the antibody and RedDot (red) for nuclear staining.
Immunohistochemistry (IHC):
AE00109 to CD79a was successfully used to stain human tonsil sections. Recommended concentration: 1-3ug/ml
Formaldehyde-fixed, paraffin-embedded human tonsil stained with CD79a Rabbit Recombinant Antibody AE00109 at 1-2ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.
Western Blot (WB):
AE00109 to CD79a was successfully used to stain an approx. 50kDa band in lysates of cell line Raji. Recommended concentration: 0.3-1ug/ml
Western Blot of a Raji lysate (30ug) stained with CD79a Rabbit Recombinant Monoclonal Antibody AE00109 at 0.5-1ug/ml (1h at ambient temp). ECL staining by HRP.
Evidence of molecular integrity:
SDS-PAGE Analysis of Purified CD79a Rabbit Recombinant Monoclonal Antibody AE00109. Confirmation of Purity and Integrity of Antibody.
Integrity of the purified antibody AE00109 under non-reduced and reduced conditions, showing intact IgG at around 130kDa (NR) and intact heavy and light chains at 50kDa and 25kDa resp. (R)
Evidence of mono-specificity:
Specificity and selectivity of AE00109 to CD79a were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed the following closely related other protein: CD79B. This related protein was part of the array used and showed no cross-reactivity signals.
Cross-reactivity assessment of CD79a Rabbit Recombinant Monoclonal Antibody AE00109 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).
