SPTBN2 Recombinant Antibody AE00290

520,00

Selective and specific mouse recombinant antibody clone rSPTBN2/1778 to human SPTBN2

Official Symbol: SPTBN2

GeneID: 6712

Swiss-Prot: O15020

Description

SPTBN2

All names and symbols: Beta-III spectrin; Spectrin beta chain, non-erythrocytic 2; Spinocerebellar ataxia 5 protein; GTRAP41; KIAA0302; SCA5; SCAR14

Host speciesMouse
ClonalityRecombinant
IsotypeIgG1, kappa
Confirmed species reactivityHuman
Confirmed applicationsPA, WB
Aeonian Rating®92
RRIDAB_2819082

Western Blot (WB):

AE00290 to SPTBN2 was successfully used to stain 170+250kDa bands in lysates of cell line HeLa. Recommended concentration: 1-3ug/ml

WB of human cell line HeLa stained with SPTBN2 Recombinant Mouse Monoclonal Antibody AE00290
HeLa with AE00290

Western Blot of HeLa lysate (30ug) stained with SPTBN2 Mouse Recombinant Antibody AE00290 at 1ug/ml (1h at ambient temp). ECL staining by HRP.

Evidence of mono-specificity (in native PA):

Specificity and selectivity of AE00290 to SPTBN2 were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed the following closely related proteins: SPTB and SPTBN1. SPTB was part of the array used and showed no cross-reactivity signals.

AE00290 on protein array
AE00290 on HuProt(TM) array

Cross-reactivity assessment of SPTBN2 Mouse Recombinant Antibody AE00290 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified SPTBN2 Mouse Recombinant Antibody AE00290. Confirmation of Purity and Integrity of Antibody.

Coomassie-stained AE00290
AE00290 Coomassie bands

Integrity of the purified antibody AE00290 under non-reduced and reduced conditions, showing intact IgG at around 150kDa (NR) and intact heavy and light chains at 48kDa and 26kDa resp. (R).

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet

Material Safety Data Sheet

Additional information

Purification

By Protein G from bioreactor concentrate

Immunogen

Recombinant fragment around aa 356-475 of human SPTBN2 protein (exact sequence is proprietary)

Epitope

Within aa 356-475 region

Cellular localization

cytoskeleton

Positive Controls

HeLa

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