CTNNB1 Recombinant Antibody AE00114

520,00

Selective and specific rabbit recombinant antibody clone CTNNB1/2030R to human CTNNB1

See all our CTNNB1 antibodies

Official Symbol: CTNNB1

GeneID: 1499

Swiss-Prot: P35222

Description

CTNNB1

All names and symbols: Armadillo; b-Catenin; Beta-catenin; Catenin beta 1; Catenin beta-1; CTNNB; EVR7; MRD19; OK/SW-cl.35; PRO2286

Host speciesRabbit
ClonalityRecombinant
IsotypeIgG
Confirmed species reactivityHuman
Confirmed applicationsICC, IHC, PA, WB
Aeonian Rating®90
RRIDAB_2736825

Immunocytochemistry (ICC):

AE00114 to CTNNB1 was successfully used to stain plasma membranes in cell line HeLa. Recommended concentration: 1-3ug/ml

HeLa with AE00114

Confocal microscopy of HeLa stained with CTNNB1 Rabbit Recombinant Antibody AE00114 at 1-2ug/ml (1h at ambient temp). Detection by CF488 (green) for the antibody and RedDot (red) for nuclear staining.

Immunohistochemistry (IHC):

AE00114 to CTNNB1 was successfully used to stain human tonsil and cervical carcinoma sections. Recommended concentration: 1-3ug/ml

IHC of human tonsil stained with CTNNB1 Rabbit Recombinant Monoclonal Antibody AE00114
Human tonsil with AE00114

Formaldehyde-fixed, paraffin-embedded human tonsil stained with CTNNB1 Recombinant Rabbit Antibody AE00114 at 1-2ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human cervical carcinoma stained with CTNNB1 Rabbit Recombinant Monoclonal Antibody AE00114
Human cervical carcinoma with AE00114

Formaldehyde-fixed, paraffin-embedded human cervical carcinoma stained with CTNNB1 Recombinant Rabbit Antibody AE00114 at 1-2ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

Western Blot (WB):

AE00114 to CTNNB1 was successfully used to stain an approx. 80kDa band in lysates of cell line HeLa. Recommended concentration: 0.3-1ug/ml

WB of HeLa stained with CTNNB1 Rabbit Recombinant Monoclonal Antibody AE00114
HeLa with AE00114

Western Blot of a HeLa lysate (30ug) stained with CTNNB1 Rabbit Recombinant Antibody AE00114 at 0.5-1ug/ml (1h at ambient temp). ECL staining by HRP.

Evidence of mono-specificity (in native PA):

Specificity and selectivity of AE00114 to CTNNB1 were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed the following related other proteins: CTNNG and ARMC4. These were part of the array used and showed no cross-reactivity signals.

AE00114 on protein array
AE00114 on HuProt(TM) array

Cross-reactivity assessment of CTNNB1 Rabbit Recombinant Antibody AE00114 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified CTNNB1 Rabbit Recombinant Antibody AE00114. Confirmation of Purity and Integrity of Antibody

Coomassie-stained AE00114
AE00114 Coomassie bands

Integrity of the purified antibody AE00114 under non-reduced and reduced conditions, showing intact IgG at around 110kDa (NR) and intact heavy and light chains at 50kDa and 25kDa resp. (R)

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant full-length human beta-Catenin protein

Epitope

Unknown

Cellular localization

cell surface, cellular junctions, nucleus, plasma membrane

Positive Controls

breast carcinoma, HeLa, MCF7

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