IgG4 Recombinant Antibody AE00117

520,00

Selective and specific rabbit recombinant monoclonal antibody clone IGHG4/2042R to human IGHG4

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Official Symbol: IGHG4

GeneID: 3503

Swiss-Prot: P01861

Description

IgG4 (G4m marker)

All names and symbols: heavy chain constant region of IgG4; Ig gamma-4 chain C region; Immunoglobulin gamma 4; Immunoglobulin heavy constant gamma 4; IgG4; IgG4-Fc

Host speciesRabbit
ClonalityRecombinant
IsotypeIgG
Confirmed species reactivityHuman
Confirmed applicationsIHC, PA
Aeonian Rating®90
RRIDAB_2736828

Immunohistochemistry (IHC):

AE00117 to IgG4 was successfully used to stain human tonsil sections. Recommended concentration: 0.3-1ug/ml

IHC of human tonsil stained with IgG4 Rabbit Recombinant Monoclonal Antibody AE00117
Human tonsil with AE00117

Formaldehyde-fixed, paraffin-embedded human tonsil stained with IgG4 Recombinant Rabbit Antibody AE00117 at 0.5-1ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH9 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

Evidence of mono-specificity (in native PA):

Specificity and selectivity of AE00117 to IgG4 were tested against >19,000 full-length human proteins on a human protein array. H. sapiens expresses the following related other proteins: IGHG1, IGHG2, IGHG3. The proteins IGHG1 and IGHG3 were part of the array used and showed an order of magnitude lower cross-reactivity signals.

AE00117 on protein array
AE00117 on HuProt(TM) array

Cross-reactivity assessment of IgG4 Rabbit Recombinant Antibody AE00117 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified IgG4 Rabbit Recombinant Antibody AE00117. Confirmation of Purity and Integrity of Antibody.

Coomassie-stained AE00117
AE00117 Coomassie bands

Integrity of the purified antibody AE00117 to IgG4 under non-reduced and reduced conditions, showing intact IgG at around 100kDa (NR) and intact heavy and light chains at 50kDa and 25kDa resp. (R)

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant full-length human heavy chain constant region of IgG4 protein

Epitope

Unknown

Cellular localization

extracellular space, plasma membrane, secreted

Positive Controls

tonsil

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