Description
BCL2
All names and symbols: Apoptosis regulator BCL-2; B-cell CLL/lymphoma-2; BCL2; PPP1R50
| Host species | Mouse |
| Clonality | Recombinant |
| Isotype | IgG1, kappa |
| Confirmed species reactivity | Human |
| Confirmed applications | IHC, PA, WB |
| Aeonian Rating® | 90 |
| RRID | AB_2736888 |
Immunohistochemistry (IHC):
AE00104 to BCL2 was successfully used to stain cells primarily in the germinal centre of human spleen sections. Recommended concentration: 0.3-1ug/ml
Formaldehyde-fixed, paraffin-embedded human spleen stained with BCL2 Mouse Recombinant Antibody AE00104 at 0.5-1ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH9 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.
Western Blot (WB):
AE00104 to BCL2 was successfully used to stain an approx. 26kDa band in lysates of cell lines A431, MCF7 and U937. Recommended concentration: 0.3-1ug/ml
Western Blot of a MCF7 lysate (30ug) stained with BCL2 Mouse Recombinant Antibody AE00104 at 0.5-1ug/ml (1h at ambient temp). ECL staining by HRP.
Western Blot of A431, MCF7 and U937 lysates (30ug) stained with BCL2 Mouse Recombinant Antibody AE00104 at 0.5-1ug/ml (1h at ambient temp). ECL staining by HRP.
Evidence of mono-specificity (in native PA):
Specificity and selectivity of AE00104 to BCL2 were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed the following closely related other proteins: BCL2L1 (isf BCLX), BCL2L2 and BAX. All these were part of the array used and showed no cross-reactivity signals.
Cross-reactivity assessment of BCL2 Mouse Recombinant Antibody AE00104 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).
Evidence of molecular integrity:
SDS-PAGE Analysis of Purified BCL2 Mouse Recombinant Antibody AE00104. Confirmation of Purity and Integrity of Antibody.
Integrity of the purified antibody AE00104 under non-reduced and reduced conditions, showing intact IgG at around 150kDa (NR) and intact heavy and light chains at 50kDa and 30kDa resp. (R)
| Expiration | Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below. |
| Storage instructions | Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light. |
| Warranty | This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund. |
| Liability | This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user. |
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