UCHL1 Recombinant Antibody AE00125

$520,00

Selective and specific mouse recombinant antibody clone rUCHL1/775 to human UCHL1

Official Symbol: UCHL1

GeneID: 7345

Swiss-Prot: P09936

Description

UCHL1

All names and symbols: Neuron cytoplasmic protein 9.5; Ubiquitin carboxyl-terminal esterase L1; Ubiquitin carboxyl-terminal hydrolase isozyme L1; Ubiquitin C-terminal hydrolase L1; Ubiquitin thioesterase L1; HEL-117; HEL-S-53; NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; SPG79; Uch-L1

Host speciesMouse
ClonalityRecombinant
IsotypeIgG1, kappa
Confirmed species reactivityHuman
Confirmed applicationsICC, IHC, PA, WB
Aeonian Rating®90
RRIDAB_2736891

Immunocytochemistry (ICC):

AE00125 to UCHL1 was successfully used to stain cytoplasm in cell line T98G. Recommended concentration: 1-3ug/ml

T98G with AE00125

Confocal microscopy of T98G stained with UCHL1 Mouse Recombinant Antibody AE00125 at 1-2ug/ml (1h at ambient temp). Detection by CF488 (green) for the antibody and NucSpot (red) for nuclear staining.

Immunohistochemistry (IHC):

AE00125 to UCHL1 was successfully used to stain neuronal cell bodies in human cerebellum sections. Recommended concentration: 1-3ug/ml

IHC of human cerebellum stained with UCHL1 Mouse Recombinant Monoclonal Antibody AE00125
Human cerebellum with AE00125

Formaldehyde-fixed, paraffin-embedded human cerebellum stained with UCHL1 Mouse Recombinant Antibody AE00125 at 1-2ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

Western Blot (WB):

AE00125 to UCHL1 was successfully used to stain an approx. 26kDa band in human cerebellum lysates. Recommended concentration: 1-3ug/ml

WB of human cerebellum stained with UCHL1 Mouse Recombinant Monoclonal Antibody AE00125
Human cerebellum with AE00125

Western Blot of a human cerebellum lysate (30ug) stained with UCHL1 Mouse Recombinant Antibody AE00125 at 1ug/ml (1h at ambient temp). ECL staining by HRP.

Evidence of mono-specificity (in native PA):

Specificity and selectivity of AE00125 to UCHL1 were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed the following closely related ubiquitin carboxyl-terminal hydrolase proteins: L3, L5 and BAP1(L2). These proteins were part of the array used and showed no cross-reactivity signals.

AE00125 on protein array
AE00125 on HuProt(TM) array

Cross-reactivity assessment of UCHL1 Mouse Recombinant Antibody AE00125 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified UCHL1 Mouse Recombinant Antibody AE00125. Confirmation of Purity and Integrity of Antibody.

Coomassie-stained AE00125
AE00125 Coomassie bands

Integrity of the purified antibody AE00125 under non-reduced and reduced conditions, showing intact IgG at around 100kDa (NR) and intact heavy and light chains at 50kDa and 25kDa resp. (R)

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein G from bioreactor concentrate

Immunogen

Recombinant full-length human UCHL1 protein

Epitope

Unknown

Positive Controls

neocortex, neurons and cells of the diffuse neuroendocrine system and their tumors, T98G

Cellular localization

cytoplasm, Endoplasmatic Reticulum, ER

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