CD5 Monoclonal Antibody AE00135

$520,00

Selective and specific mouse monoclonal antibody clone CD5/2416 to human CD5

See all our CD5 antibodies

Official Symbol: CD5

GeneID: 921

Swiss-Prot: P06127

Description

CD5

All names and symbols: CD_antigen: CD5; Lymphocyte antigen T1/Leu-1; T-cell surface glycoprotein CD5; LEU1; T1

Host speciesMouse
ClonalityMonoclonal
IsotypeIgG2b, kappa



Confirmed species reactivityHuman
Confirmed applicationsICC, IHC, PA, WB
Aeonian Rating®85
RRIDAB_2736899



Immunocytochemistry (ICC):

AE00135 to CD5 was successfully used to stain plasma membranes in cell line Ramos. Recommended concentration: 1-3ug/ml.

ICC of Ramos stained with CD5 mouse Monoclonal Antibody AE00135
Ramos with AE00135

Confocal microscopy of PFA-fixed Ramos stained with CD5 Mouse Monoclonal Antibody AE00135 at 1-2ug/ml (1h at ambient temp). Detection by CF488 (green) for the antibody and RedDot (red) for nuclear staining.

Immunohistochemistry (IHC):

AE00135 to CD5 was successfully used to stain T cell membranes in human tonsil sections. Recommended concentration: 1-3ug/ml

IHC of human tonsil stained with CD5 Mouse Monoclonal Antibody AE00135
Human tonsil with AE00135

Formaldehyde-fixed, paraffin-embedded human tonsil stained with CD5 Mouse Monoclonal Antibody AE00135 at 1-2ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

Western Blot (WB):

AE00135 to CD5 was successfully used to stain an approx. 65kDa band in lysates of cell line Jurkat. Recommended concentration: 0.5-1.5ug/ml

WB of Jurkat stained with CD5 Mouse Monoclonal Antibody AE00135
Jurkat with AE00135

Western Blot of a Jurkat lysate (30ug) stained with CD5 Mouse Monoclonal Antibody AE00135 at 1ug/ml (1h at ambient temp). ECL staining by HRP.

Evidence of mono-specificity (in native PA):

Specificity and selectivity of AE00135 to CD5 was tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed no closely related other proteins.

AE00135 on protein array
AE00135 on HuProt(TM) array

Cross-reactivity assessment of CD5 Mouse Monoclonal Antibody AE00135 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified CD5 Mouse Monoclonal Antibody AE00135. Confirmation of Purity and Integrity of Antibody.

Coomassie-stained AE00135
AE00135 Coomassie bands

Integrity of the purified antibody AE00135 under non-reduced and reduced conditions, showing intact IgG at around 150kDa (NR) and intact heavy and light chains at 50kDa and 25kDa resp. (R)

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant fragment around aa 269-366 of human CD5 protein (exact sequence is proprietary)

Epitope

Extracellular domain (within aa 269-366 region)

Cellular localization

cell surface, plasma membrane

Positive Controls

lymph node, MOLT4, Ramos, tonsil

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