Glypican-3 Recombinant Antibody AE00138

$520,00

Selective and specific mouse recombinant antibody clone rGPC3/863 to human Glypican-3

Official Symbol: GPC3

GeneID: 2719

Swiss-Prot: P51654

Description

Glypican-3

All names and symbols: Glypican 3; Glypican-3; Intestinal protein OCI-5; SGBS1 inhibitor; DGSX; GPC3; GTR2-2; MXR7; OCI-5; OCI5; SDYS; SGB; SGBS

Host speciesMouse
ClonalityRecombinant
IsotypeIgG1, kappa
Confirmed species reactivityHuman
Confirmed applicationsICC, IHC, PA
Aeonian Rating®90
RRIDAB_2737009

Immunocytochemistry (ICC):

AE00138 to Glypican-3 was successfully used to stain membranes in cell line HepG2. Recommended concentration: 1-3ug/ml

HepG2 with AE00138

Confocal microscopy of methanol-fixed HepG2 stained with Glypican-3 Mouse Recombinant Antibody AE00138 at 1-2ug/ml (1h at ambient temp). Detection by CF488 (green) for the antibody and RedDot (red) for nuclear staining.

Immunohistochemistry (IHC):

AE00138 to Glypican-3 was successfully used to stain malignant cells in human hepatocellular carcinoma sections. Recommended concentration: 1-3ug/ml

IHC of human hepatocellular carcinoma stained with GPC3 Mouse Recombinant Monoclonal Antibody AE00138
Human hepatocellular carcinoma with AE00138

Formaldehyde-fixed, paraffin-embedded human hepatocellular carcinoma stained with Glypican-3 Mouse Recombinant Antibody AE00138 at 01-2ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

Evidence of mono-specificity (in native PA):

Specificity and selectivity of AE00138 to Glypican-3 were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed closely related other protein GPC5. This protein was part of the array and showed no cross-reactivity signals.

AE00138 on protein array
AE00138 on HuProt(TM) array

Cross-reactivity assessment of Glypican-3 Mouse Recombinant Antibody AE00138 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified Glypican-3 Mouse Recombinant Antibody AE00138. Confirmation of Purity and Integrity of Antibody.

Coomassie-stained AE00138
AE00138 Coomassie bands

Integrity of the purified antibody AE00138 under non-reduced and reduced conditions, showing intact IgG at around 140kDa (NR) and intact heavy and light chains at 50kDa and 25kDa resp. (R)

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet

Material Safety Data Sheet

Additional information

Purification

By Protein G from bioreactor concentrate

Immunogen

Recombinant full-length human Glypican-3 protein

Epitope

Unknown

Cellular localization

cell surface, plasma membrane, secreted

Positive Controls

hepatocellular carcinoma

Reviews

There are no reviews yet.

Only logged in customers who have purchased this product may leave a review.

SKU: AE00138 Categories: , , , Tags: , ,