S100A4 Monoclonal Antibody AE00151

$520,00

Selective and specific mouse monoclonal antibody clone S100A4/1481 to human S100A4

Official Symbol: S100A4

GeneID: 6275

Swiss-Prot: P26447

Description

S100A4

All names and symbols: Calvasculin; Metastasin; Placental calcium-binding protein; Protein Mts1; Protein S100-A4; S100 calcium binding protein A4; S100 calcium-binding protein A4; 18A2; 42A; CAPL; FSP1; MTS1; P9KA; PEL98

Host speciesMouse
ClonalityMonoclonal
IsotypeIgG1, kappa



Confirmed species reactivityHuman
Confirmed applicationsICC, IHC, PA, WB
Aeonian Rating®80
RRIDAB_2744512

Immunocytochemistry (ICC):

AE00151 to S100A was successfully used to stain nuclei and to lesser extent cytoplasm in cell line HeLa. Recommended concentration: 1-3ug/ml

HeLa with AE00151

Confocal microscopy of HeLa stained with S100A Mouse Monoclonal Antibody AE00151 at 1-2ug/ml (1h at ambient temp). Detection by CF488 (green) for the antibody and RedDot(red) for nuclear staining.



Immunohistochemistry (IHC):

AE00151 to S100A4 was successfully used to stain mesenchymal nuclei in human placenta sections. Recommended concentration: 0.3-1ug/ml

IHC of human placenta stained with S100A4 Mouse Monoclonal Antibody AE00151
Human placenta with AE00151

Formaldehyde-fixed, paraffin-embedded human placenta stained with S100A4 Mouse Monoclonal Antibody AE00151 at 0.3-0.5ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

Western Blot (WB):

AE00151 to S100A4 was successfully used to stain an approx. 12kDa band in lysates of cell lines HeLa, A549 and A375. Recommended concentration: 0.5-1.5ug/ml

WB of HeLa., A549 and A375 stained with S100A4 Mouse Monoclonal Antibody AE00151
HeLa, A549, A375 with AE00151

Western Blot of HeLa, A549 and A375 lysates (30ug) stained with S100A4 Mouse Monoclonal Antibody AE00151 at 1ug/ml (1h at ambient temp). ECL staining by HRP.

Evidence of mono-specificity (in native PA):

Specificity and selectivity of AE00151 to S100A4 were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed the following closely related proteins S100A1, 2, 3 and 6. These proteins were part of the array used and showed no cross-reactivity signals.

AE00151 on protein array
AE00151 on HuProt(TM) array

Cross-reactivity assessment of S100A4 Mouse Monoclonal Antibody AE00151 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified S100A4 Mouse Monoclonal Antibody AE00151. Confirmation of Purity and Integrity of Antibody.

Coomassie-stained AE00151
AE00151 Coomassie bands

Integrity of the purified antibody AE00151 under non-reduced and reduced conditions, showing intact IgG at around 140kDa (NR) and intact heavy and light chains at 50kDa and 25kDa resp. (R)

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein G from bioreactor concentrate

Immunogen

Recombinant fragment of human S100A4 protein (exact sequence is proprietary)

Epitope

Unknown

Cellular localization

exosomes, nucleus, secreted

Positive Controls

A375, A549, HeLa, placenta

Reviews

There are no reviews yet.

Only logged in customers who have purchased this product may leave a review.

SKU: AE00151 Categories: , , Tags: , , ,