NSE Monoclonal Antibody AE00170

$520,00

Selective and specific mouse monoclonal antibody clone ENO2/1462 to human NSE

Official Symbol: ENO2

GeneID: 2026

Swiss-Prot: P09104

Description

NSE

All names and symbols: 2-phospho-D-glycerate hydro-lyase; Enolase 2; Gamma-enolase; Neural Enolase; Neuron-Specific Enolase; ENO2; HEL-S-279; NSE

Host speciesMouse
ClonalityMonoclonal
IsotypeIgG2b, kappa



Confirmed species reactivityHuman, Mouse, Rat
Confirmed applicationsIHC, PA
Aeonian Rating®85
RRIDAB_2750957



Immunohistochemistry (IHC):

AE00170 to NSE was successfully used to stain malignancy in human pheochromocytoma sections and islets of Langerhans in mouse pancreas sections. Recommended concentration: 0.1-0.3ug/ml

IHC of human pheochromocytoma stained with NSE Mouse Monoclonal Antibody AE00170
Human pheochromocytoma with AE00170

Formaldehyde-fixed, paraffin-embedded human pheochromocytoma stained with NSE Mouse Monoclonal Antibody AE00170 at 0.1-0.2ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of mouse pancreas stained with NSE Mouse Monoclonal Antibody AE00170
Mouse pancreas with AE00170

Formaldehyde-fixed, paraffin-embedded mouse pancreas stained with NSE Mouse Monoclonal Antibody AE00170 at 0.1-0.2ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

Evidence of mono-specificity (in native PA):

Specificity and selectivity of AE00170 to NSE were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed no closely related other proteins.

AE00170 on protein array
AE00170 on HuProt(TM) array

Cross-reactivity assessment of NSE Mouse Monoclonal Antibody AE00170 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified NSE Mouse Monoclonal Antibody AE00170. Confirmation of Purity and Integrity of Antibody.

Coomassie-stained AE00170
AE00170 Coomassie bands

Integrity of the purified antibody AE00170 under non-reduced and reduced conditions, showing intact IgG at around 150kDa (NR) and intact heavy and light chains at 50kDa and 25kDa resp. (R)

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant fragment around aa 416-433 of human NSE protein (exact sequence is proprietary)

Epitope

C-terminus (within aa 416-433 region)

Cellular localization

cytoplasm

Positive Controls

cerebellum, HeLa, HepG2, pancreas, pheochromocytoma, SHSY5Y, Y79

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