Factor XIIIa Monoclonal Antibody AE00174

520,00

Selective and specific mouse monoclonal antibody clone F13A1/1448 to human Factor XIIIa

Official Symbol: F13A1

GeneID: 2162

Swiss-Prot: P00488

Description

Factor XIIIa

All names and symbols: Coagulation factor XIIIa; Factor XIII-A; Factor XIIIa; Coagulation factor XIII A chain; Protein-glutamine gamma-glutamyltransferase A chain; Transglutaminase A chain; F13A1; F13A

Host speciesMouse
ClonalityMonoclonal
IsotypeIgG2b, kappa



Confirmed species reactivityHuman
Confirmed applicationsIHC, PA, WB
Aeonian Rating®83
RRIDAB_2750961



Immunohistochemistry (IHC):

AE00174 to Factor XIIIa was successfully used to stain extracellular in human histiocytoma sections. Recommended concentration: 1-3ug/ml

IHC of human histiocytoma stained with Factor XIIIa1 Mouse Monoclonal Antibody AE00174
Human histiocytoma with AE00174

Formaldehyde-fixed, paraffin-embedded human histiocytoma stained with Factor XIIIa Mouse Monoclonal Antibody AE00174 at 1-2ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

Western Blot (WB):

AE00174 to Factor XIIIa was successfully used to stain an approx. 90kDa band in lysates of cell line HeLa. Recommended concentration: 0.3-1ug/ml

WB of HeLa stained with F13A1 Mouse Monoclonal Antibody AE00174
HeLa with AE00174

Western Blot of HeLa lysate (30ug) stained with Factor XIIIa Mouse Monoclonal Antibody AE00174 at 1ug/ml (1h at ambient temp). ECL staining by HRP.

Evidence of mono-specificity (in native PA):

Specificity and selectivity of AE00174 to Factor XIIIa were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed no closely related other proteins.

AE00174 on protein array
AE00174 on HuProt(TM) array

Cross-reactivity assessment of Factor XIIIa Mouse Monoclonal Antibody AE00174 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified Factor XIIIa Mouse Monoclonal Antibody AE00174. Confirmation of Purity and Integrity of Antibody.

Coomassie-stained AE00174
AE00174 Coomassie bands

Integrity of the purified antibody AE00174 under non-reduced and reduced conditions, showing intact IgG at around 160+150kDa (NR) and intact heavy and light chains at 50kDa and 25kDa resp. (R). Double band explained by degree of glycosylation.

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant fragment around aa 46-181 of human F13A1 protein (exact sequence is proprietary)

Epitope

N terminus (within aa 46-181 region)

Cellular localization

cytoplasm, secreted

Positive Controls

HeLa, histiocytoma

Reviews

There are no reviews yet.

Only logged in customers who have purchased this product may leave a review.

SKU: AE00174 Categories: , , Tags: , ,