CD25 Monoclonal Antibody AE00185

$520,00

Selective and specific mouse monoclonal antibody clone IL2RA/2395 to human CD25

Official Symbol: IL2RA

GeneID: 3559

Swiss-Prot: P01589

Description

CD25

All names and symbols: CD_antigen: CD25; Interleukin 2 receptor subunit alpha; Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2R subunit alpha; TAC antigen; CD25; IDDM10; IL2R; IL2RA; IL-2-RA; IMD41; TCGFR; p55

Host speciesMouse
ClonalityMonoclonal
IsotypeIgG2b, kappa



Confirmed species reactivityHuman
Confirmed applicationsIHC, PA
Aeonian Rating®83
RRIDAB_2750973



Immunohistochemistry (IHC):

AE00185 to CD25 was successfully used to stain macrophages in human tonsil sections. Recommended concentration: 1-3ug/ml

IHC of human tonsil stained with CD25 Mouse Monoclonal Antibody AE00185
Human tonsil with AE00185

Formaldehyde-fixed, paraffin-embedded human tonsil stained with CD25 Mouse Monoclonal Antibody AE00185 at 1-2ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH9 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

Evidence of mono-specificity (in native PA):

Specificity ans selectivity of AE00185 to CD25 were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed no closely related other proteins.

AE00185 on protein array
AE00185 on HuProt(TM) array

Cross-reactivity assessment of CD25 Mouse Monoclonal Antibody AE00185 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified CD25 Mouse Monoclonal Antibody AE00185. Confirmation of Purity and Integrity of Antibody.

Coomassie-stained AE00185
AE00185 Coomassie bands

Integrity of the purified antibody AE00185 under non-reduced and reduced conditions, showing intact IgG at around 150kDa (NR) and intact heavy and light chains at 50kDa and 25kDa resp. (R)

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant fragment around aa 42-183 of human CD25 protein (exact sequence is proprietary)

Epitope

Extracellular domain (within aa 42-183 region)

Cellular localization

cell surface, plasma membrane

Positive Controls

lymph node, PHA-stimulated lymphocytes, tonsil

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