MMP2 Monoclonal Antibody AE00192

$520,00

Selective and specific mouse monoclonal antibody clone MMP2/1501 to human MMP2

Official Symbol: MMP2

GeneID: 4313

Swiss-Prot: P08253

Description

MMP2

All names and symbols: 72 kDa gelatinase; 72 kDa type IV collagenase; Matrix metalloproteinase-2; Gelatinase A; matrix metallopeptidase 2; CLG4; CLG4A; MMP-2; MMP2; MMP-II; MONA; TBE-1; PEX

Host speciesMouse
ClonalityMonoclonal
IsotypeIgG1, kappa



Confirmed species reactivityHuman
Confirmed applicationsPA, WB
Aeonian Rating®80
RRIDAB_2752237



Western Blot (WB):

AE00192 to MMP2 was successfully used to stain an approx. 70kDa band in lysates of cell line U87MG Recommended concentration: 0.5-1.5ug/ml

WB of human U87MG stained with MMP2 Mouse Monoclonal Antibody AE00192
Human U87MG with AE00192

Western Blot of a U87MG lysate (right) (30ug) stained with MMP2 Mouse Monoclonal Antibody AE00192 at 1ug/ml (1h at ambient temp). ECL staining by HRP.

Evidence of mono-specificity (in native PA):

Specificity and selectivity of AE00192 to MMP2 were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed no closely related other proteins.

AE00192 on protein array
AE00192 on HuProt(TM) array

Cross-reactivity assessment of MMP2 Mouse Monoclonal Antibody AE00192 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified MMP2 Mouse Monoclonal Antibody AE00192. Confirmation of Purity and Integrity of Antibody.

Coomassie-stained AE00192
AE00192 Coomassie bands

Integrity of the purified antibody AE00192 under non-reduced and reduced conditions, showing intact IgG at around 150kDa (NR) and intact heavy and light chains at 50kDa and 25kDa resp. (R)

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein G from bioreactor concentrate

Immunogen

Recombinant fragment around aa 444-575 of human MMP2 protein (exact sequence is proprietary)

Epitope

Within aa 444-575 region

Cellular localization

extracellular space

Positive Controls

U87MG

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