MTAP Monoclonal Antibody AE00193

$520,00

Selective and specific mouse monoclonal antibody clone MTAP/1813 to human MTAP

Official Symbol: MTAP

GeneID: 4507

Swiss-Prot: Q13126

Description

MTAP

All names and symbols: 5′-methylthioadenosine phosphorylase; methylthioadenosine phosphorylase; MTA phosphorylase; S-methyl-5′-thioadenosine phosphorylase; BDMF; c86fus; DMSFH; DMSMFH; HEL-249; LGMBF; MSAP; MTAP; MTAPase

Host speciesMouse
ClonalityMonoclonal
IsotypeIgG2b, kappa



Confirmed species reactivityHuman
Confirmed applicationsIHC, PA, WB
Aeonian Rating®80
RRIDAB_2752238



Immunohistochemistry (IHC):

AE00193 to MTAP was successfully used to stain epithelial cells in human kidney sections. Recommended concentration: 1-3ug/ml

IHC of human kidney stained with MTAP Mouse Monoclonal Antibody AE00193
Human kidney with AE00193

Formaldehyde-fixed, paraffin-embedded human kidney stained with MTAP Mouse Monoclonal Antibody AE00193 at 1-2ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

Western Blot (WB):

AE00193 to MTAP was successfully used to stain an approx. 30kDa band in lysates of cell lines HeLa, A431, HepG2 and HAP1. Recommended concentration: 0.5-1.5ug/ml

HeLa, A431, HepG2 and HAP-1 with AE00193

Western Blot of HeLa, A431, HepG2 and HAP1 lysates (30ug) stained with MTAP Mouse Monoclonal Antibody AE00193 at 1ug/ml (1h at ambient temp). ECL staining by HRP.

Evidence of mono-specificity (in native PA):

Specificity and selectivity of AE00193 to MTAP were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed no closely related other proteins.

AE00193 on protein array
AE00193 on HuProt(TM) array

Cross-reactivity assessment of MTAP Mouse Monoclonal Antibody AE00193 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified MTAP Mouse Monoclonal Antibody AE00193. Confirmation of Purity and Integrity of Antibody.

Coomassie-stained AE00193
AE00193 Coomassie bands

Integrity of the purified antibody AE00193 under non-reduced and reduced conditions, showing intact IgG at around 160kDa (NR) and intact heavy and light chains at 50kDa and 25kDa resp. (R)

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant fragment around aa 97-196 of human MTAP protein (exact sequence is proprietary)

Epitope

Within aa 97-196 region

Cellular localization

cytosol, nucleus

Positive Controls

A431, HAP1, HeLa, HepG2, kidney

Reviews

There are no reviews yet.

Only logged in customers who have purchased this product may leave a review.

SKU: AE00193 Categories: , , Tags: , ,