GCDFP-15 Monoclonal Antibody AE00198

$520,00

Selective and specific mouse monoclonal antibody clone PIP/1571 to human GCDFP-15

Official Symbol: PIP

GeneID: 5304

Swiss-Prot: P12273

Description

GCDFP-15

All names and symbols: Gross cystic disease fluid protein-15; Prolactin induced protein; Prolactin-induced protein; Prolactin-inducible protein; Secretory actin-binding protein; GCDFP15; GCDFP-15; GPIP4; gp17; PIP; SABP

Host speciesMouse
ClonalityMonoclonal
IsotypeIgG2a, kappa



Confirmed species reactivityHuman
Confirmed applicationsIHC, PA, WB
Aeonian Rating®80
RRIDAB_2753339



Immunohistochemistry (IHC):

AE00198 to GCDFP-15 was successfully used to stain epithelial cells in human breast carcinoma sections. Recommended concentration: 0.3-1ug/ml

IHC of human breast carcinoma stained with GCDFP-15 Mouse Monoclonal Antibody AE00198
Human breast carcinoma with AE00198

Formaldehyde-fixed, paraffin-embedded human breast carcinoma stained with GCDFP-15 Mouse Monoclonal Antibody AE00198 at 0.5-1ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH9 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

Western Blot (WB):

AE00198 to GCDFP-15 was successfully used to stain an approx. 18kDa band in lysates of human pancreas and of cell line HepG2. Recommended concentration: 0.5-1.5ug/ml

WB of human pancreas and hepG2 stained with GCDFP-15 Mouse Monoclonal Antibody AE00198
Human pancreas and HepG2 with AE00198

Western Blot of human pancreas (left) and HepG2 (right) lysates (30ug) stained with GCDFP-15 Mouse Monoclonal Antibody AE00198 at 1ug/ml (1h at ambient temp). ECL staining by HRP.

Evidence of mono-specificity (in native PA):

Specificity and selectivity of AE00198 to GCDFP-15 were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed no closely related other proteins.

AE00198 on protein array
AE00198 on HuProt(TM) array

Cross-reactivity assessment of GCDFP-15 Mouse Monoclonal Antibody AE00198 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified GCDFP-15 Mouse Monoclonal Antibody AE00198. Confirmation of Purity and Integrity of Antibody.

Coomassie-stained AE00198
AE00198 Coomassie bands

Integrity of the purified antibody AE00198 under non-reduced and reduced conditions, showing intact IgG at around 160kDa (NR) and intact heavy and light chains at 55kDa and 26kDa resp. (R)

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant fragment around aa 41-146 of human GCDFP-15 protein (exact sequence is proprietary)

Epitope

Within aa 41-146 region

Cellular localization

extracellular space, secreted

Positive Controls

breast, HepG2, pancreas

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