CD27 Monoclonal Antibody AE00205

$520,00

Selective and specific mouse monoclonal antibody clone LPFS2/1611 to human CD27

Official Symbol: CD27

GeneID: 939

Swiss-Prot: P26842

Description

CD27

All names and symbols: CD_antigen: CD27; CD27 antigen; T-cell activation antigen CD27; Tumor necrosis factor receptor superfamily member 7; CD27L receptor; T14; TNFRSF7; S152; Tp55; S152. LPFS2

Host speciesMouse
ClonalityMonoclonal
IsotypeIgG1, kappa



Confirmed species reactivityHuman
Confirmed applicationsICC, IHC, PA
Aeonian Rating®80
RRIDAB_2813759



Immunocytochemistry (ICC):

AE00205 to CD27 was successfully used to stain plasma membranes in cell line Ramos. Recommended concentration: 1 3ug/ml

ICC of Ramos stained with CD27 Mouse Monoclonal Antibody AE00205
Ramos with AE00205

Confocal microscopy of Ramos stained with CD27 Mouse Monoclonal Antibody AE00205 at 1-2ug/ml (1h at ambient temp). Detection by CF488 (green) for the antibody and RedDot (red) for nuclear staining.

Immunohistochemistry (IHC):

AE00205 to CD27 was successfully used to stain T-cell membranes around the germinal centre in human tonsil sections and intraepithelial T-cells in human colon sections. Recommended concentration: 1-3ug/ml

IHC of human tonsil stained with CD27 Mouse Monoclonal Antibody AE00205
Human tonsil with AE00205

Formaldehyde-fixed, paraffin-embedded human tonsil stained with CD27 Mouse Monoclonal Antibody AE00205 at 1-2ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH9 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human colon stained with CD27 Mouse Monoclonal Antibody AE00205
Human colon with AE00205

Formaldehyde-fixed, paraffin-embedded human colon stained with CD27 Mouse Monoclonal Antibody AE00205 at 1-2ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH9 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

Evidence of mono-specificity (in native PA):

Specificity and selectivity of AE00205 to CD27 were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed no closely related proteins.

AE00205 on protein array
AE00205 on HuProt(TM) array

Cross-reactivity assessment of CD27 Mouse Monoclonal Antibody AE00205 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified CD27 Mouse Monoclonal Antibody AE00205. Confirmation of Purity and Integrity of Antibody.

Coomassie-stained AE00205
AE00205 Coomassie bands

Integrity of the purified antibody AE00205 under non-reduced and reduced conditions, showing intact IgG at around 150kDa (NR) and intact heavy and light chains at 50kDa and 24kDa resp. (R)

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein G from bioreactor concentrate

Immunogen

Recombinant full length human CD27 protein

Epitope

Unknown

Cellular localization

cell surface, plasma membrane

Positive Controls

colon, Ramos, tonsil

Reviews

There are no reviews yet.

Only logged in customers who have purchased this product may leave a review.

SKU: AE00205 Categories: , , , , , Tags: , ,