TP53-pS6 Polyclonal Antibody AE00222

520,00825,00

Phospho-specific rabbit polyclonal antibody to human TP53, phosphorylated S6 (TP53-pS6)

Official Symbol: TP53

GeneID: 7157

Swiss-Prot: PP04637

Description

TP53-pS6

All names and symbols: Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor protein p53; Tumor suppressor p53; BCC7; LFS1; P53; TRP53

Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Confirmed species reactivityHuman
Confirmed applicationsWB
Aeonian Rating®70
RRIDAB_2813808

Western Blot (WB):

AE00222 to TP53-pS6 was successfully used to stain an approx. 55kDa band in lysates of HeLa after UV radiation. Recommended concentration: 1-3ug/ml

WB of HeLa stained with TP53-pS6 Rabbit Phospho-Specific Polyclonal Antibody AE00222
HeLa with AE00222

Western Blot of 30ug HeLa lysate with or without stimulation by UV radiation, stained with TP53-pS6 Rabbit Polyclonal Antibody AE00222 at 1ug/ml (1h at ambient temp). ECL staining by HRP.

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

Antigen-affinity purified from serum by sequential phospho-peptide and non-phospho-peptide affinity columns

Immunogen

A synthetic peptide around phosphorylated S6 of human TP53 (NP_000537.3)

Epitope

A short sequence around phosphorylated S6 (exact sequence is proprietary)

Cellular localization

nucleus

Positive Controls

UV-treated HeLa

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