HSPB1-pS78 Polyclonal Antibody AE00231

520,00825,00

Phospho-specific rabbit polyclonal antibody to HSP27, phosphorylated S78 (HSPB1-pS78)

See all our HSP27 antibodies

Official Symbol: HSPB1

GeneID: 3315

Swiss-Prot: P04792

Description

HSPB1-pS78

All names and symbols: Heat Shock Protein family B (small) member 1; Heat shock protein beta-1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; Stress-Responsive Protein 27; CMT2F; HMN2B; HSP27; HSP 27; HSP28; Hsp25; HSPB1; SRP27; HS.76067; HEL-S-102

Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Confirmed species reactivityHuman
Confirmed applicationsWB
Aeonian Rating®70
RRIDAB_2814727

Western Blot (WB):

AE00231 to HSPB1-pS78 was successfully used to stain an approx. 27kDa band in lysates of UV-treated, serum-starved or Anisomycin-treated HeLa. Recommended concentration: 1-3ug/ml

WB of HeLa stained with HSPB1-pS78 Rabbit Phospho-Specific Polyclonal Antibody AE00231
HeLa with AE00231

Western Blots of 30ug lysates of HeLa with and without UV radiation (15-30 min), or serum-starved (overnight) HeLa with or without Anisomycin (25ug/ml, 30min), stained with HSPB1-pS78 Rabbit Polyclonal Antibody AE00231 at 1ug/ml (1h at ambient temp). ECL staining by HRP.

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

Antigen-affinity purified from serum by sequential phospho-peptide and non-phospho-peptide affinity columns

Immunogen

A synthetic peptide around phosphorylated S78 of human HSP27 (NP_001531.1)

Epitope

A short sequence around phosphorylated S78 (exact sequence is proprietary)

Cellular localization

cell surface, cytoskeleton, plasma membrane

Positive Controls

Anisomycin-treated HeLa, UV-treated HeLa

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