KRT19 Recombinant Antibody AE00253

$520,00

Selective and specific mouse recombinant antibody clone rKRT19/799 to human KRT19

Official Symbol: KRT19

GeneID: 3880

Swiss-Prot: P08727

Description

KRT19

All names and symbols: Cytokeratin 19; Cytokeratin-19; Keratin-19; Keratin, type I cytoskeletal 19; CK19; CK-19; K19; K1CS; KRT19

Host speciesMouse
ClonalityRecombinant
IsotypeIgG1, kappa
Confirmed species reactivityHuman
Confirmed applicationsIHC, PA, WB
Aeonian Rating®90
RRIDAB_2734751

Immunohistochemistry (IHC):

AE00253 to KRT19 was successfully used to stain epithelial cells in human colon carcinoma sections. Recommended concentration: 1-3ug/ml

IHC of human colon carcinoma stained with KRT19 Mouse Recombinant Monoclonal Antibody AE00253
Human colon cacrinoma with AE00253

Formaldehyde-fixed, paraffin-embedded human colon carcinoma stained with KRT19 Mouse Recombinant Antibody AE00253 at 1-2ug/ml for 30 minutes at RT. Epitope retrieval: boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

Western Blot (WB):

AE00253 to KRT19 was successfully used to stain an approx. 45kDa band in lysates of cell line HepG2. Recommended concentration: 1-3ug/ml

WB of HepG2 stained with KRT19 Mouse Recombinant Monoclonal Antibody AE00253
HepG2 with AE00253

Western Blot of HepG2 lysate (30ug) stained with KRT19 Mouse Recombinant Antibody AE00253 at 1ug/ml (1h at ambient temp). ECL staining by HRP.

Evidence of mono-specificity (in native PA):

Specificity and selectivity of AE00253 to KRT19 were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed many closely related members of the KRT family. Most if not all KRT proteins are part of the array used and showed no cross-reactivity signals.

AE00253 on protein array
AE00253 on HuProt(TM) array

Cross-reactivity assessment of KRT19 Mouse Recombinant Antibody AE00253 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified KRT19 Mouse Recombinant Antibody AE00253. Confirmation of Purity and Integrity of Antibody.

Coomassie-stained AE00253
AE00253 Coomassie bands

Integrity of the purified antibody AE00253 under non-reduced and reduced conditions, showing intact IgG at around 100kDa (NR) and intact heavy and light chains at 50kDa and 28kDa resp. (R).

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein G from bioreactor concentrate

Immunogen

Recombinant full-length human KRT19 protein

Epitope

Unknown

Cellular localization

cytoplasm, cytoskeleton

Positive Controls

carcinoma, HeLa, HepG2

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