Description
HSP60
All names and symbols: 60 kDa heat shock protein; Chaperonin 60; Heat shock protein 60; Heat shock protein family D (Hsp60) member ;, mitochondrial 60 kDa chaperonin; Mitochondrial matrix protein P1; P60 lymphocyte protein; CPN60; GROEL; HLD4; HSP-60; HSP60; HSP65; HSPD1; HuCHA60; SPG13
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1, kappa |
| Confirmed species reactivity | Human |
| Confirmed applications | ICC, IHC, PA, WB |
| Aeonian Rating® | 80 |
| RRID | AB_2891215 |
Immunocytochemistry (ICC):
AE00293 to HSP60 was successfully used to stain mitochondria in cell lines HeLa and MCF7. Recommended concentration: 1-3ug/ml
Confocal microscopy of HeLa stained with HSP60 Mouse Monoclonal Antibody AE00293 at 1-2ug/ml (1h at ambient temp). Detection by CF488 (green) for the antibody and RedDot (red) for nuclear staining.
Confocal microscopy of MCF7 stained with HSP60 Mouse Monoclonal Antibody AE00293 at 1-2ug/ml (1h at ambient temp). Detection by CF488 (green) for the antibody and RedDot (red) for nuclear staining.
Immunohistochemistry (IHC):
AE00293 to HSP60 was successfully used to stain mitochondria in human carcinoma sections of the bladder, lung and kidney. Recommended concentration: 1-3ug/ml
Formaldehyde-fixed, paraffin-embedded human bladder carcinoma stained with HSP60 Mouse Monoclonal Antibody AE00293 at 1-2ug/ml for 30 minutes at RT. Epitope retrieval: Heating at 95°C for 45 min at pH9 followed by 20 min cooling. DAB staining by HRP polymer.
Western Blot (WB):
AE00293 to HSP60 was successfully used to stain an approx. 60kDa band in lysates of cell line HeLa. Recommended concentration: 1-3ug/ml
Western Blot of a human HeLa lysate (30ug) stained with HSP60 Mouse Monoclonal Antibody AE00293 at 1-2ug/ml (1h at ambient temp). ECL staining by HRP.
Evidence of mono-specificity (in native PA):
Specificity and selectivity of AE00293 to HSP60 were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed no closely related proteins.
Cross-reactivity assessment of HSP60 Mouse Monoclonal Antibody AE00293 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).
| Expiration | Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below. |
| Storage instructions | Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light. |
| Warranty | This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund. |
| Liability | This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user. |
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