EGFR-pY1148 Polyclonal Antibody AE00308

520,00825,00

Phospho-specific rabbit polyclonal antibody to EGFR, phosphorylated Y1148 (EGFR-pY1148)

Official Symbol: EGFR

GeneID: 1956

Swiss-Prot: P00533

Description

EGFR-pY1048

All names and symbols: Epithelial Growth Factor Receptor; EGFR; ERBB; ERBB1; HER1; NISBD2; PIG61; mENAa

Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Confirmed species reactivityHuman, Mouse, Rat
Confirmed applicationsICC, WB
Aeonian Rating®70
RRIDAB_2892798

Immunocytochemistry (ICC):

AE00308 to EGFR-pY1048 was successfully used to stain nuclear membranes in LPS-treated cell line HepG2. Recommended concentration: 5-15ug/ml

ICC of LPS-treated HepG2 stained with EGFR-pY1148 rabbit Polyclonal Antibody AE00308
LPS-treated HepG2 with AE00308

Confocal microscopy of PFA-fixed, and LPS-treated for 4h, HepG2 (0.1% TX100 permeabilised) stained with EGFR-pY1148 Rabbit Polyclonal Antibody AE00308 at 5ug/ml (1h at 37℃). Detection by AF594 (red) for the EGFR-pY1148 rabbit antibody, AF488 (green) for beta-tubulin mouse antibody, and DAPI (blue) for nuclear staining.

Western Blot (WB):

AE00308 to EGFR-pY1048 was successfully used to stain an approx. 160kDa band in mouse and rat liver lysates. Recommended concentration: 1-3ug/ml

WB of mouse and rat liver stained with EGFR-pY1148 Rabbit Polyclonal Antibody AE00308
Mouse and rat liver with AE00308

Western Blot of 10ug liver lysates from mouse (lanes 1 and 2) and rat (lanes 3 and 4) stained with EGFR-pY1048 Rabbit Polyclonal Antibody AE00308 at 1ug/ml (overnight at 4℃) in the presence (+) or absence (-) of its antigen (P peptide) or its non-phosphorylated control (C peptide). ECL staining by HRP.

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

Antigen-affinity purified from serum by sequential phospho-peptide and non-phospho-peptide affinity columns

Immunogen

A synthetic peptide around phosphorylated Y1172 of human EGFR (NP_005219.2) corresponding to Y1148 of the mature protein (starts with L25).

Epitope

A short sequence around phosphorylated Y1148 (exact sequence is proprietary)

Cellular localization

cell surface, plasma membrane, translocation to nucleus upon EGF stimulation

Positive Controls

LPS-treated HepG2, rat liver

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