CD137 Recombinant Antibody AE00310

520,00

Functional and specific mouse recombinant antibody clone r4B4-1 to human CD137

See all our CD137 antibodies

Official Symbol: TNFRSF9

GeneID: 3604

Swiss-Prot: Q07011

Description

CD137

All names and symbols: Tumor necrosis factor receptor superfamily member 9; TNF receptor superfamily member 9; 4-1BB ligand receptor; T-cell antigen 4-1BB homolog; T-cell antigen ILA; 4-1BB; CD137; CDw137; ILA; TNFRSF9

Host speciesMouse
ClonalityRecombinant
IsotypeIgG1, kappa, recombinant version of clone 4B4-1 (4B4-1-1)
Confirmed species reactivityHuman
Confirmed applicationsFC, Monocyte activation, NK cell induction
Aeonian Rating®85
RRIDAB_2904495

Immunoassays

Flow Cytometry (FC):

The original clone 4B4-1 was successfully used in Flow Cytometry on tumor-infiltrating CD8 T cells (CD8TIL).

Duhen T, Duhen R, Montler R, Moses J, Moudgil T, de Miranda NF, Goodall CP, Blair TC, Fox BA, McDermott JE, Chang SC, Grunkemeier G, Leidner R, Bell RB, Weinberg AD. Co-expression of CD39 and CD103 identifies tumor-reactive CD8 T cells in human solid tumors. Nat Commun. 2018 Jul 13;9(1):2724. doi: 10.1038/s41467-018-05072-0. PMID: 30006565.

Biological Activity

Binding to activated T-cells:

The original clone 4B4-1-1 (in later publications described as 4B4-1) successfully showed good binding affinity for Con A-activated T cells. The antibody also showed suppression of lymphocyte proliferation in vitro. Binding of the antibody to the lymphocyte receptor 4-1BB (CD137) was confirmed by Flow Cytometry and ELISA.

Kim et al. Immunoregulatory effects of a monoclonal antibody to human 4-1 bb molecule on alloantigen-mediated immune responses. Proceedings of the Korean Society of Applied Pharmacology (1995), pg 82-82.

Monocyte activation:

The original clone 4B4-1 was successfully used to activate human peripheral blood monocytes by binding to the CD137.

Kienzle G, von Kempis J. CD137 (ILA/4-1BB), expressed by primary human monocytes, induces monocyte activation and apoptosis of B lymphocytes. Int Immunol. 2000 Jan;12(1):73-82. doi: 10.1093/intimm/12.1.73. PMID: 10607752.

Competition for binding with another CD137 monoclonal antibody:

The original clone 4B4-1 was successfully used to compete for binding to CD137 with CD137 mAb M121.

Kienzle G, von Kempis J. CD137 (ILA/4-1BB), expressed by primary human monocytes, induces monocyte activation and apoptosis of B lymphocytes. Int Immunol. 2000 Jan;12(1):73-82. doi: 10.1093/intimm/12.1.73. PMID: 10607752.

Induction of IFN-y in NK cells:

The original clone 4B4-1 was successfully used to induce production of IFN-y in NK cells by binding to CD137.

Baessler T, Charton JE, Schmiedel BJ, Grünebach F, Krusch M, Wacker A, Rammensee HG, Salih HR. CD137 ligand mediates opposite effects in human and mouse NK cells and impairs NK-cell reactivity against human acute myeloid leukemia cells. Blood. 2010 Apr 15;115(15):3058-69. doi: 10.1182/blood-2009-06-227934. Erratum in: Blood. 2010 Dec 23;116(26):6152. PMID: 20008791.

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

A fusion protein of human CD137 (NP_001552.2) and Glutathione-S-Transferase (GST)

Epitope

Unknown

Cellular localization

cell surface, plasma membrane

Positive Controls

conA-activated T-cells, NK cells, tumor-infiltrated CD8 T cells

clonality

recombinant

Isotype

IgG1

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