CD137 Recombinant Antibody AE00310

Description

CD137

All names and symbols: Tumor necrosis factor receptor superfamily member 9; TNF receptor superfamily member 9; 4-1BB ligand receptor; T-cell antigen 4-1BB homolog; T-cell antigen ILA; 4-1BB; CD137; CDw137; ILA; TNFRSF9

Host species	Mouse
Clonality	Recombinant
Isotype	IgG1, kappa, recombinant version of clone 4B4-1 (4B4-1-1)

Confirmed species reactivity	Human
Confirmed applications	FC, Monocyte activation, NK cell induction
Aeonian Rating®	85
RRID	AB_2904495

Immunoassays

Flow Cytometry (FC):

The original clone 4B4-1 was successfully used in Flow Cytometry on tumor-infiltrating CD8 T cells (CD8TIL).

Duhen T, Duhen R, Montler R, Moses J, Moudgil T, de Miranda NF, Goodall CP, Blair TC, Fox BA, McDermott JE, Chang SC, Grunkemeier G, Leidner R, Bell RB, Weinberg AD. Co-expression of CD39 and CD103 identifies tumor-reactive CD8 T cells in human solid tumors. Nat Commun. 2018 Jul 13;9(1):2724. doi: 10.1038/s41467-018-05072-0. PMID: 30006565.

Biological Activity

Binding to activated T-cells:

The original clone 4B4-1-1 (in later publications described as 4B4-1) successfully showed good binding affinity for Con A-activated T cells. The antibody also showed suppression of lymphocyte proliferation in vitro. Binding of the antibody to the lymphocyte receptor 4-1BB (CD137) was confirmed by Flow Cytometry and ELISA.

Kim et al. Immunoregulatory effects of a monoclonal antibody to human 4-1 bb molecule on alloantigen-mediated immune responses. Proceedings of the Korean Society of Applied Pharmacology (1995), pg 82-82.

Monocyte activation:

The original clone 4B4-1 was successfully used to activate human peripheral blood monocytes by binding to the CD137.

Kienzle G, von Kempis J. CD137 (ILA/4-1BB), expressed by primary human monocytes, induces monocyte activation and apoptosis of B lymphocytes. Int Immunol. 2000 Jan;12(1):73-82. doi: 10.1093/intimm/12.1.73. PMID: 10607752.

Competition for binding with another CD137 monoclonal antibody:

The original clone 4B4-1 was successfully used to compete for binding to CD137 with CD137 mAb M121.

Kienzle G, von Kempis J. CD137 (ILA/4-1BB), expressed by primary human monocytes, induces monocyte activation and apoptosis of B lymphocytes. Int Immunol. 2000 Jan;12(1):73-82. doi: 10.1093/intimm/12.1.73. PMID: 10607752.

Induction of IFN-y in NK cells:

The original clone 4B4-1 was successfully used to induce production of IFN-y in NK cells by binding to CD137.

Baessler T, Charton JE, Schmiedel BJ, Grünebach F, Krusch M, Wacker A, Rammensee HG, Salih HR. CD137 ligand mediates opposite effects in human and mouse NK cells and impairs NK-cell reactivity against human acute myeloid leukemia cells. Blood. 2010 Apr 15;115(15):3058-69. doi: 10.1182/blood-2009-06-227934. Erratum in: Blood. 2010 Dec 23;116(26):6152. PMID: 20008791.

Expiration	Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructions	Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
Warranty	This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
Liability	This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.