Description
CD137L (mouse)
All names and symbols: Tumor necrosis factor ligand superfamily member 9; tumor necrosis factor (ligand) superfamily, member 9; CD137 ligand; 4-1BB ligand; 4-1BBL; 4-1BB-L; AI848817; Cd137l; Cd157l; Ly63l; Ly63l
| Host species | Rat |
| Clonality | Recombinant |
| Isotype | IgG2a, kappa, recombinant version of clone TKS-1 |
| Confirmed species reactivity | Mouse |
| Confirmed applications | FC, blocking |
| Aeonian Rating® | 100 |
| RRID | AB_2904501 |
Immunoassays
Flow Cytometry (FC):
The original clone TKS-1 was successfully used on murine cell lines and on splenic activated T cells.
Futagawa T, Akiba H, Kodama T, Takeda K, Hosoda Y, Yagita H, Okumura K. Expression and function of 4-1BB and 4-1BB ligand on murine dendritic cells. Int Immunol. 2002 Mar;14(3):275-86. doi: 10.1093/intimm/14.3.275. PMID: 11867564.
The original clone TKS-1 was successfully used on murine LPS-activated bone marrow derived dendritic cells.
Mbanwi AN, Lin GHY, Wang KC, Watts TH. Constitutive interaction between 4-1BB and 4-1BBL on murine LPS-activated bone marrow dendritic cells masks detection of 4-1BBL by TKS-1 but not 19H3 antibody. J Immunol Methods. 2017 Nov;450:81-89. doi: 10.1016/j.jim.2017.08.001. PMID: 28789924.
Biological Activity
Blocking CD137/CD137L signalling in dendritic cells:
The original clone TKS-1 was successfully used to block CD137/CD137L signalling in splenic dendritic cells.
Futagawa T, Akiba H, Kodama T, Takeda K, Hosoda Y, Yagita H, Okumura K. Expression and function of 4-1BB and 4-1BB ligand on murine dendritic cells. Int Immunol. 2002 Mar;14(3):275-86. doi: 10.1093/intimm/14.3.275. PMID: 11867564.
Partially blocking CD137/CD137L signalling in co-cultured astrocytes/microglia:
The original clone TKS-1 was successfully used to partially block CD137/CD137L signalling in lipid-laden astrocytes seeded with TKS-1 pretreated microglia.
Kim J, Kwon YH, Kim CS, Tu TH, Kim BS, Joe Y, Chung HT, Goto T, Kawada T, Park T, Choi MS, Kim MS, Yu R. The involvement of 4-1BB/4-1BBL signaling in glial cell-mediated hypothalamic inflammation in obesity. FEBS Open Bio. 2018 Apr 19;8(5):843-853. doi: 10.1002/2211-5463.12426. PMID: 29744298.
Blocking the CD137 binding site in activated dendritic cells:
The original clone TKS-1 was successfully used to block the CD137 binding site in murine LPS-activated bone marrow dendritic cells.
Mbanwi AN, Lin GHY, Wang KC, Watts TH. Constitutive interaction between 4-1BB and 4-1BBL on murine LPS-activated bone marrow dendritic cells masks detection of 4-1BBL by TKS-1 but not 19H3 antibody. J Immunol Methods. 2017 Nov;450:81-89. doi: 10.1016/j.jim.2017.08.001. PMID: 28789924.
| Expiration | Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below. |
| Storage instructions | Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light. |
| Warranty | This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund. |
| Liability | This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user. |
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