CD5 Recombinant Antibody AE00351

$520,00$1.500,00

Specific, and tissue microarray-validated rabbit recombinant antibody clone AE005R to human CD5

See all our CD5 antibodies

Official Symbol: CD5

GeneID: 921

Swiss-Prot: P06127

For BSA-free at 1mg IgG/ml, please ask us for a quotation.

Description

CD5

All names and symbols: CD_antigen: CD5; Lymphocyte antigen T1/Leu-1; T-cell surface glycoprotein CD5; LEU1; T1

Host speciesRabbit
ClonalityRecombinant
IsotypeIgG
Confirmed species reactivityHuman
Confirmed applicationsIHC
Aeonian Rating®93
RRIDAB_2916355

Immunohistochemistry (IHC):

AE00351 to CD5 was successfully used to stain plasma membranes of T-cells in human lymph node, thymus and tonsil sections. Recommended concentration: 1-3ug/ml

IHC of human lymph node stained with CD5 Rabbit Recombinant Antibody AE00351
Human lymph node with AE00351

Formaldehyde-fixed, paraffin-embedded human lymph node stained with CD5 Rabbit Recombinant Antibody AE00351 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human thymus stained with CD5 Rabbit Recombinant Antibody AE00351
Human thymus with AE00351

Formaldehyde-fixed, paraffin-embedded human thymus stained with CD5 Rabbit Recombinant Antibody AE00351 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human tonsil stained with CD5 Rabbit Recombinant Antibody AE00351
Human tonsil with AE00351

Formaldehyde-fixed, paraffin-embedded human tonsil stained with CD5 Rabbit Recombinant Antibody AE00351 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

Tissue Microarray Validation:

Normal tissues

Strong positiveWeak to moderateNo staining at all
lymph nodeappendix mucosaadipose
thymusbreastadrenal gland
tonsilbronchial glandsaorta, media
colon mucosaappendix muscular wall
duodenum mucosabone marrow
endocervixbronchus mucosa
ectocervixcerebellum
endometriumcerebrum
esophagus squamous epitheliumcolon muscular wall
fallopian tubeduodenum Brunner gland
gallbladderepididymis
ileum mucosaheart muscle
liverkidney
pancreaslung
rectum mucosamyometrium
sinus paranasalisovarian stroma
skinparathyroid gland
spleenparotid gland
stomachpituitary gland
urotheliumplacenta
pregnant uterus
prostate
seminal vesicle
skeletal muscle
testis
thyroid gland
urinary bladder muscular wall

Cancer tissues

Strong positiveWeak to moderateNo staining at all
chronic lymphocytic leukemia in lymph nodecervical squamous cell carcinomaperipheral T-cell lymphoma in lymph node
Hodgkin’s lymphomaclear cell renal cell carcinoma
mantle cell lymphomacolorectal adenocarcinoma
diffuse large B-cell lymphoma
papillary renal cell carcinoma
seminoma
ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant fragment within the aa 32-372 region of human CD5 protein

Epitope

Extracellular domain within aa 32-372 region

Cellular localization

cell surface, plasma membrane

Positive Controls

lymph node, spleen, thymus, tonsil

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