Description
CD34
All names and symbols: CD_antigen: CD34; CD34 molecule; Hematopoietic progenitor cell antigen CD34; CD34
| Host species | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Confirmed species reactivity | Human |
| Confirmed applications | IHC |
| Aeonian Rating® | 90 |
| RRID | AB_2916359 |
Immunohistochemistry (IHC):
AE00355 to CD34 was successfully used to stain membranes of endothelial cells in human endometrium, kidney, and pituitary gland sections. Recommended concentration: 1-3ug/ml
Formaldehyde-fixed, paraffin-embedded human endometrium stained with CD34 Rabbit Recombinant Antibody AE00355 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Formaldehyde-fixed, paraffin-embedded human kidney stained with CD34 Rabbit Recombinant Antibody AE00355 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Formaldehyde-fixed, paraffin-embedded human pituitary gland stained with CD34 Rabbit Recombinant Antibody AE00355 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Tissue Microarray Validation:
Normal tissues
| Strong positive | Weak to moderate | No staining at all |
|---|---|---|
| adrenal gland | adipose | hepatocytes |
| aorta, media | appendix mucosa | |
| bronchus mucosa | appendix muscular wall | |
| colon mucosa | bone marrow | |
| colon muscular wall | breast | |
| duodenum Brunner gland | cerebellum | |
| endocervix | cerebrum | |
| endometrium | duodenum mucosa | |
| epididymis | ectocervix | |
| fallopian tube | esophagus squamous epithelium | |
| kidney | gallbladder | |
| lung | heart muscle | |
| myometrium | ileum mucosa | |
| pancreas | liver portal vessels and sinusoidal endothelial cells | |
| parathyroid gland | lymph node | |
| parotid gland | ovarian stroma | |
| pituitary gland | placenta early | |
| placenta mature | prostate | |
| seminal vesicle | rectum mucosa | |
| testis | sinus paranasalis | |
| thymus | skeletal muscle | |
| thyroid gland | skin | |
| urinary bladder muscular wall | spleen | |
| stomach | ||
| tonsil | ||
| pregnant uterus | ||
| urothelium |
Cancer tissues
| Strong positive | Weak to moderate | No staining at all |
|---|---|---|
| angiosarcoma | angiomyolipoma | |
| dermatofibrosarcoma protuberans | myxofibrosarcoma bloodvessels | |
| Kaposi sarcoma | primitive neuroectodermal tumor bloodvessels | |
| Expiration | Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below. |
| Storage instructions | Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light. |
| Warranty | This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund. |
| Liability | This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user. |
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