Melan A Recombinant Antibody AE00375

$520,00$1.500,00

Specific, and tissue microarray-validated mouse recombinant antibody clone AE900M to human Melan A

Official Symbol: MLANA

GeneID: 2315

Swiss-Prot: Q16655

For BSA-free at 1mg IgG/ml, please ask us for a quotation.

Description

Melan A

All names and symbols: Antigen LB39-AA; Antigen SK29-AA; Melanoma antigen recognized by T-cells 1; Melan-A; MelanA; Melan A; MART1; MART-1; MLANA

Host speciesMouse
ClonalityRecombinant
IsotypeIgG2a, kappa
Confirmed species reactivityHuman
Confirmed applicationsIHC
Aeonian Rating®90
RRIDAB_2921276

Immunohistochemistry (IHC):

AE00375 to Melan A was successfully used to stain melanocytes in healthy skin, non-neoplastic melanocytes in Melan A-negative basal cell carcinoma, and tumour cells in melanoma sections. Recommended concentration: 1-3 μg/ml

IHC of human skin stained with Melan A Rabbit Recombinant Antibody AE00375
Human skin with AE00375

Formaldehyde-fixed, paraffin-embedded human skin stained with Melan A Mouse Recombinant Antibody AE00375 at 1-2 μg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human basal cell carcinoma stained with Melan A Rabbit Recombinant Antibody AE00375
Human basal cell carcinoma with AE00375

Formaldehyde-fixed, paraffin-embedded human basal cell carcinoma stained with Melan A Mouse Recombinant Antibody AE00375 at 1-2 μg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of human melanoma stained with Melan A Rabbit Recombinant Antibody AE00375
Human melanoma with AE00375

Formaldehyde-fixed, paraffin-embedded human melanoma stained with Melan A Mouse Recombinant Antibody AE00375 at 1-2 μg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

Tissue Microarray Validation:

Normal tissues

Strong positiveWeak to moderateNo staining at all
skinadipose
adrenal gland
aorta media
appendix mucosa
appendix muscular wall
bone marrow
breast
bronchus mucosa
cerebellum
cerebrum
colon mucosa
colon muscular wall
duodenum Brunner gland
duodenum mucosa
ectocervix
endocervix
endometrium
epididymis
esophagus squamous epithelium
fallopian tube
gallbladder
heart muscle
kidney
liver
lung
myometrium
ovarian stroma
pancreas
parathyroid gland
parotid gland
pituitary gland
placenta
pregnant uterus
prostate
rectum mucosa
seminal vesicle
sinus paranasalis
skeletal muscle
spleen
stomach
testis
thymus
thyroid gland
urinary bladder muscular wall
urothelium

Cancer tissues

Strong positiveWeak to moderateNo staining at all
melanomaadrenocortical adenoma
adrenocortical carcinoma
basal cell carcinoma
clear cell renal cell carcinoma
colorectal adenocarcinoma
endometrioid ovarian carcinoma
high-grade serous carcinoma
pancreas ductal adenocarcinoma
pharynx squamous cell carcinoma
prostate adenocarcinoma
renal oncocytoma
skin squamous cell carcinoma
urothelial carcinoma
ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant full length human Melan A protein

Epitope

Unknown

Cellular localization

Endoplasmatic Reticulum, Golgi, melanosome

Positive Controls

melanoma, skin

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