Description
Melan A
All names and symbols: Antigen LB39-AA; Antigen SK29-AA; Melanoma antigen recognized by T-cells 1; Melan-A; MelanA; Melan A; MART1; MART-1; MLANA
| Host species | Mouse |
| Clonality | Recombinant |
| Isotype | IgG2a, kappa |
| Confirmed species reactivity | Human |
| Confirmed applications | IHC |
| Aeonian Rating® | 90 |
| RRID | AB_2921276 |
Immunohistochemistry (IHC):
AE00375 to Melan A was successfully used to stain melanocytes in healthy skin, non-neoplastic melanocytes in Melan A-negative basal cell carcinoma, and tumour cells in melanoma sections. Recommended concentration: 1-3 μg/ml
Formaldehyde-fixed, paraffin-embedded human skin stained with Melan A Mouse Recombinant Antibody AE00375 at 1-2 μg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Formaldehyde-fixed, paraffin-embedded human basal cell carcinoma stained with Melan A Mouse Recombinant Antibody AE00375 at 1-2 μg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Formaldehyde-fixed, paraffin-embedded human melanoma stained with Melan A Mouse Recombinant Antibody AE00375 at 1-2 μg/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Tissue Microarray Validation:
Normal tissues
| Strong positive | Weak to moderate | No staining at all |
|---|---|---|
| skin | adipose | |
| adrenal gland | ||
| aorta media | ||
| appendix mucosa | ||
| appendix muscular wall | ||
| bone marrow | ||
| breast | ||
| bronchus mucosa | ||
| cerebellum | ||
| cerebrum | ||
| colon mucosa | ||
| colon muscular wall | ||
| duodenum Brunner gland | ||
| duodenum mucosa | ||
| ectocervix | ||
| endocervix | ||
| endometrium | ||
| epididymis | ||
| esophagus squamous epithelium | ||
| fallopian tube | ||
| gallbladder | ||
| heart muscle | ||
| kidney | ||
| liver | ||
| lung | ||
| myometrium | ||
| ovarian stroma | ||
| pancreas | ||
| parathyroid gland | ||
| parotid gland | ||
| pituitary gland | ||
| placenta | ||
| pregnant uterus | ||
| prostate | ||
| rectum mucosa | ||
| seminal vesicle | ||
| sinus paranasalis | ||
| skeletal muscle | ||
| spleen | ||
| stomach | ||
| testis | ||
| thymus | ||
| thyroid gland | ||
| urinary bladder muscular wall | ||
| urothelium |
Cancer tissues
| Strong positive | Weak to moderate | No staining at all |
|---|---|---|
| melanoma | adrenocortical adenoma | |
| adrenocortical carcinoma | ||
| basal cell carcinoma | ||
| clear cell renal cell carcinoma | ||
| colorectal adenocarcinoma | ||
| endometrioid ovarian carcinoma | ||
| high-grade serous carcinoma | ||
| pancreas ductal adenocarcinoma | ||
| pharynx squamous cell carcinoma | ||
| prostate adenocarcinoma | ||
| renal oncocytoma | ||
| skin squamous cell carcinoma | ||
| urothelial carcinoma |
| Expiration | Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below. |
| Storage instructions | Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light. |
| Warranty | This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund. |
| Liability | This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user. |
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