Description
CD1a
All names and symbols: CD1a molecule; CD_antigen: CD1a; T-cell surface glycoprotein CD1a; T-cell surface antigen T6/Leu-6; hTa1 thymocyte antigen; CD1; FCB6; HTA1; R4; T6
| Host species | Mouse |
| Clonality | Recombinant |
| Isotype | IgG1, kappa |
| Confirmed species reactivity | Human |
| Confirmed applications | IHC |
| Aeonian Rating® | 90 |
| RRID | AB_2916352 |
Immunohistochemistry (IHC):
AE00348 to CD1a was successfully used to stain Langerhans cells in human skin and tonsil sections, and cortical lymphocyte membranes in thymus sections. Recommended concentration: 1-3ug/ml
Formaldehyde-fixed, paraffin-embedded human skin stained with CD1a Mouse Recombinant Antibody AE00348 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Formaldehyde-fixed, paraffin-embedded human tonsil stained with CD1a Mouse Recombinant Antibody AE00348 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Formaldehyde-fixed, paraffin-embedded human thymus stained with CD1a Mouse Recombinant Antibody AE00348 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.
Tissue Microarray Validation:
Normal tissues
| Strong positive | Weak to moderate | No staining at all |
|---|---|---|
| skin, Langerhans cells | ectocervix, Langerhans cells | adipose |
| thymus cortex | esophagus squamous epithelium | adrenal gland |
| tonsil squamous epithelium, Langerhans cells | aorta, media | |
| appendix mucosa | ||
| appendix muscular wall | ||
| bone marrow | ||
| breast | ||
| bronchus mucosa | ||
| cerebellum | ||
| cerebrum | ||
| colon | ||
| duodenum | ||
| endocervix | ||
| endometrium | ||
| epididymis | ||
| fallopian tube | ||
| gallbladder | ||
| heart muscle | ||
| kidney | ||
| lung | ||
| lymph node | ||
| myometrium | ||
| ovarian stroma | ||
| pancreas | ||
| parathyroid | ||
| parotid gland | ||
| pituitary gland | ||
| pregnant uterus | ||
| placenta | ||
| prostate | ||
| rectal mucosa | ||
| seminal vesicle | ||
| spleen | ||
| stomach | ||
| testis | ||
| thymus medulla | ||
| thyroid gland | ||
| tonsil | ||
| urinary bladder muscular wall | ||
| urothelium |
Cancer tissues
| Strong positive | Weak to moderate | No staining at all |
|---|---|---|
| Diffuse Large B Cell Lymphoma, dendritic cells | Hodgkin’s lymphoma, dendritic cells | |
| lymphoblastic T-cell lymphoma |
| Expiration | Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below. |
| Storage instructions | Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light. |
| Warranty | This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund. |
| Liability | This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user. |
Reviews
There are no reviews yet.