CD1a Recombinant Antibody AE00242

520,00

Selective and specific rabbit recombinant antibody clone C1A/1506R to human CD1a

See all our CD1a antibodies

Official Symbol: CD1A

GeneID: 909

Swiss-Prot: P06126

Description

CD1a

All names and symbols: CD1a molecule; CD_antigen: CD1a; T-cell surface glycoprotein CD1a; T-cell surface antigen T6/Leu-6; hTa1 thymocyte antigen; CD1; FCB6; HTA1; R4; T6

Host speciesRabbit
ClonalityRecombinant
IsotypeIgG
Confirmed species reactivityHuman
Confirmed applicationsIHC, PA
Aeonian Rating®90
RRIDAB_2814954

Immunohistochemistry (IHC):

AE00242 to CD1a was successfully used to stain Langerhans cells in human skin sections. Recommended concentration: 1-3ug/ml

IHC of human skin stained with CD1a Rabbit Recombinant Monoclonal Antibody AE00242
Human skin with AE00242

Formaldehyde-fixed, paraffin-embedded human skin stained with CD1a Rabbit Recombinant Antibody AE00242 at 1-2ug/ml for 30 minutes at RT. Epitope retrieval: boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

Evidence of mono-specificity (in native PA):

Specificity and selectivity of AE00242 to CD1a were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed the following closely related other proteins: CD1b, c, d and e. The related proteins CD1b, c and e were part of the array used and showed no cross-reactivity signals.

AE00242 on protein array
AE00242 on HuProt(TM) array

Cross-reactivity assessment of CD1a Rabbit Recombinant Antibody AE00242 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified CD1a Rabbit Recombinant Antibody AE00242. Confirmation of Purity and Integrity of Antibody.

Coomassie-stained AE00242
AE00242 Coomassie bands

Integrity of the purified antibody AE00242 under non-reduced and reduced conditions, showing intact IgG at around 120kDa (NR) and intact heavy and light chains at 50kDa and 25kDa resp. (R).

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant full-length human CD1a protein

Epitope

Unknown

Cellular localization

cell surface, endosomes, plasma membrane

Positive Controls

skin

clonality

recombinant

host species

Rabbit

Isotype

IgG

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