Description
Ki-67
All names and symbols: Antigen identified by monoclonal antibody Ki-67; Antigen KI-67; Antigen Ki67; Marker of proliferation Ki-67; Proliferation marker protein Ki-67; KIA; MIB-; MIB-1; MKI67; PPP1R105
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b, kappa |
| Confirmed species reactivity | Human |
| Confirmed applications | ICC, IHC, PA, WB |
| Aeonian Rating® | 80 |
| RRID | AB_2752235 |
Immunocytochemistry (ICC):
AE00190 to Ki-67 was successfully used to stain nucleoli in cell line HeLa. Recommended concentration: 1-3ug/ml
Confocal microscopy of HeLa stained with Ki-67 Mouse Monoclonal Antibody AE00190 at 1-2ug/ml (1h at ambient temp). Detection by CF488 (green) for the antibody and Phalloidin (red) for membrane staining.
Immunohistochemistry (IHC):
AE00190 to Ki-67 was successfully used to stain proliferating lymphocytes in human tonsil sections and of active basal layer cells of human epidermis sections. Recommended concentration: 1-3ug/ml
Formaldehyde-fixed, paraffin-embedded human tonsil stained with Ki-67 Mouse Monoclonal Antibody AE00190 at 1-2ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.
Formaldehyde-fixed, paraffin-embedded human skin stained with Ki-67 Mouse Monoclonal Antibody AE00190 at 1-2ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.
Western Blot (WB):
AE00190 to Ki-67 was successfully used to stain approx. 200kDa bands in lysates of cell line Raji and Ramos. Recommended concentration: 0.3-1ug/ml
Western Blot of a Raji and Ramos lysates (30ug) stained with Ki-67 Mouse Monoclonal Antibody AE00190 at 0.3-0.6ug/ml (1h at ambient temp). ECL staining by HRP.
Evidence of mono-specificity (in native PA):
Specificity and selectivity of AE00190 to Ki-67 were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed no closely related other proteins.
Cross-reactivity assessment of Ki-67 Mouse Monoclonal Antibody AE00190 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).
Evidence of molecular integrity:
SDS-PAGE Analysis of Purified Ki-67 Mouse Monoclonal Antibody AE00190. Confirmation of Purity and Integrity of Antibody.
Integrity of the purified antibody AE00190 under non-reduced and reduced conditions, showing intact IgG at around 150kDa (NR) and intact heavy and light chains at 55kDa and 25kDa resp. (R)
| Expiration | Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below. |
| Storage instructions | Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light. |
| Warranty | This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund. |
| Liability | This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user. |
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