CELA3B Recombinant Antibody AE00358

520,001.500,00

Specific, and tissue microarray-validated mouse recombinant antibody clone AE410M to human CELA3B

Official Symbol: CELA3B

GeneID: 23436

Swiss-Prot: P08861

For BSA-free at 1mg IgG/ml, please ask us for a quotation.

Description

CELA3B

All names and symbols: chymotrypsin like elastase 3B; Chymotrypsin-like elastase family member 3B; Elastase IIIB; Elastase-3B; Protease E; CBPP; ELA3B

Host speciesMouse
ClonalityRecombinant
IsotypeIgG1, kappa
Confirmed species reactivityHuman
Confirmed applicationsIHC
Aeonian Rating®91
RRIDAB_2920704

Immunohistochemistry (IHC):

AE00358 to CELA3B was successfully used to stain acinar cells in human panceas, acinar cell carcinoma, and at the luminal epithelium surface in appendix sections. Recommended concentration: 1-3ug/ml

IHC of pancreas stained with CELA3B Rabbit Recombinant Antibody AE00358
Human pancreas with AE00358

Formaldehyde-fixed, paraffin-embedded human pancreas stained with CELA3B Mouse Recombinant Antibody AE00358 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of acinar carcinoma stained with CELA3B Rabbit Recombinant Antibody AE00358
Human acinar carcinoma with AE00358

Formaldehyde-fixed, paraffin-embedded human acinar carcinoma stained with CELA3B Mouse Recombinant Antibody AE00358 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of appendix stained with CELA3B Rabbit Recombinant Antibody AE00358
Human appendix with AE00358

Formaldehyde-fixed, paraffin-embedded human appendix stained with CELA3B Mouse Recombinant Antibody AE00358 at 1-2ug/ml for 60 minutes at 37°C. Epitope retrieval: Autoclave at 121°C, pH7.8 for 5 min followed by 20 min cooling. DAB staining by HRP polymer.

Tissue Microarray Validation:

Normal tissues

Strong positiveWeak to moderateNo staining at all
pancreasappendix mucosaadipose
ileum mucosaadrenal gland
tonsil surface epitheliumaorta, media
appendix muscular wall
bone marrow
breast
bronchus glands
bronchus mucosa
cerebellum
cerebrum
colon mucosa
colon muscular wall
duodenum Brunner gland
duodenum mucosa
ectocervix
endocervix
endometrium
epididymis
esophagus squamous epithelium
fallopian tube
gallbladder
heart muscle
kidney
liver
lung
lymph node
myometrium
ovarian stroma
parathyroid gland
parotid gland
pituitary gland
placenta
pregnant uterus
prostate
rectum mucosa
seminal vesicle
sinus paranasalis
skeletal muscle
skin
spleen
stomach
testis
thymus 
thyroid gland
urinary bladder muscular wall
urothelium

Cancer tissues

Strong positiveWeak to moderateNo staining at all
acinar cell carcinomabreast cancer no special type
cholangiocarcinoma
clear cell renal cell carcinoma
colorectal adenocarcinoma
endometrial cancer
esophagus adenocarcinoma
gastric adenocarcinoma
gastrointestinal stromal tumor
high-grade serous carcinoma
larynx squamous cell carcinoma
liposarcoma
lung adenocarcinoma
medullary thyroid cancer
pancreas ductal adenocarcinoma
pancreas neuroendocrine tumor
papillary thyroid cancer
pheochromocytoma
prostate adenocarcinoma
urothelial carcinoma
vulva squamous cell carcinoma
ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant fragment within the aa 82-238 region of human CELA3B protein

Epitope

Within the aa 82-238 region

Cellular localization

secreted

Positive Controls

acinar carcinoma, pancreas

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