Description
EGFR-pY992
All names and symbols: Epithelial Growth Factor Receptor; EGFR; ERBB; ERBB1; HER1; NISBD2; PIG61; mENA
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Confirmed species reactivity | Human, Mouse |
| Confirmed applications | WB |
| Aeonian Rating® | 70 |
| RRID | AB_2813795 |
Western Blot (WB):
AE00213 to EGFR-pY992 was successfully used to stain an approx. 180kDa band in lysates of A431 and NIH3T3 after overnight serum starvation and subsequent treatment with EGF. Recommended concentration: 1-3ug/ml
Western Blot of 30ug lysate from serum-starved A431 with or without stimulation by 100mg/ml EGF (30 min, 37C) ), stained with EGFR-pY992 Rabbit Polyclonal Antibody AE00213 at 1ug/ml (1h at ambient temp). ECL staining by HRP.
Western Blot of 30ug lysate from serum-starved NIH3T3 with or without stimulation by 100mg/ml EGF (30 min, 37C) ), stained with EGFR-pY992 Rabbit Polyclonal Antibody AE00213 at 1ug/ml (1h at ambient temp). ECL staining by HRP.
| Expiration | Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below. |
| Storage instructions | Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light. |
| Warranty | This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund. |
| Liability | This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user. |
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