CD79a Recombinant Antibody AE00109

520,00

Selective and specific rabbit recombinant antibody clone IGA/1790R to human CD79a

See all our CD79a antibodies

Official symbol: CD79A

GeneID: 973

Swiss-Prot: P11912

Description

CD79a (aa202-216)

All names and symbols: B-cell antigen receptor complex-associated protein alpha chain; CD_antigen: CD79a; Ig-alpha; MB-1 membrane glycoprotein; Membrane-bound immunoglobulin-associated protein; Surface IgM-associated protein; CD79a; IGA; MB1; MB-1

Host speciesRabbit
ClonalityRecombinant
IsotypeIgG
Species reactivityHuman
Confirmed applicationsICC, IHC, PA, WB
Aeonian Rating®100
RRIDAB_2734785

Immunocytochemistry (ICC):

AE00109 to CD79a was successfully used to stain vesicles in cell line Raji. Recommended concentration: 1-3ug/ml.

Raji with AE00109

Confocal microscopy of PFA-fixed Raji stained with CD79a Rabbit Recombinant Antibody AE00109 at 1-2ug/ml (1h at ambient temp). Detection by CF488 (green) for the antibody and RedDot (red) for nuclear staining.

Immunohistochemistry (IHC):

AE00109 to CD79a was successfully used to stain human tonsil sections. Recommended concentration: 1-3ug/ml

IHC of human tonsil stained with CD79a Rabbit Recombinant Monoclonal Antibody AE00109
Human tonsil with AE00109

Formaldehyde-fixed, paraffin-embedded human tonsil stained with CD79a Rabbit Recombinant Antibody AE00109 at 1-2ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

Western Blot (WB):

AE00109 to CD79a was successfully used to stain an approx. 50kDa band in lysates of cell line Raji. Recommended concentration: 0.3-1ug/ml

WB of Raji stained with CD79a Rabbit Recombinant Monoclonal Antibody AE00109
Raji with AE00109

Western Blot of a Raji lysate (30ug) stained with CD79a Rabbit Recombinant Antibody AE00109 at 0.5-1ug/ml (1h at ambient temp). ECL staining by HRP.

Evidence of mono-specificity (in native PA):

Specificity and selectivity of AE00109 to CD79a were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed the following closely related other protein: CD79B. This related protein was part of the array used and showed no cross-reactivity signals.

AE00109 on protein array
AE00109 on HuProt(TM) array

Cross-reactivity assessment of CD79a Rabbit Recombinant Antibody AE00109 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified CD79a Rabbit Recombinant Antibody AE00109. Confirmation of Purity and Integrity of Antibody.

Coomassie-stained AE00109
AE00109 Coomassie bands

Integrity of the purified antibody AE00109 under non-reduced and reduced conditions, showing intact IgG at around 130kDa (NR) and intact heavy and light chains at 50kDa and 25kDa resp. (R)

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Synthetic peptide GTYQDVGSLNIADVQ corresponding to aa 202-216 of human CD79a protein

Epitope

aa 202-216

Cellular localization

cell surface, plasma membrane

Positive Controls

Daudi, Germinal Center B-cells in lymph node, Ramos, spleen, tonsil

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