Cat nr AE00109 Recombinant Rabbit Monoclonal Antibody, clone IGA/1790R to:
CD79a, CD79a molecule
IGA, MB1, MB-1, B-cell antigen receptor complex-associated protein alpha chain, Ig-alpha, MB-1 membrane glycoprotein, Membrane-bound immunoglobulin-associated protein, Surface IgM-associated protein, CD_antigen: CD79a

Cellular localization
Plasma membrane, endosomes
Official Symbol (Gene)
CD79A
GeneID
973
Swiss-Prot
Applications
IHC, ICC, WB
Positive controls
Daudi, Ramos, Germinal Center B-cells in lymph node, spleen and tonsil.
Aeonian Rating®
100
RRID
Isotype Rabbit IgG
Species reactivity Human
Immunogen Synthetic peptide GTYQDVGSLNIADVQ corresponding to aa 202-216 of human CD79a protein
Epitope aa 202-216
Purification By Protein A from bioreactor concentrate
Formulations 200ug IgG/ml in PBS, 0.05% BSA, 0.05% azide (20ug or 100ug)
1mg IgG/ml in PBS (100ug, or contact us)
Storage instructions Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20C or -80C and keep one aliquot at 4C for daily experimentations. Azide will preserve antibody at 4C for 6-12 months, when kept away from direct sun light.
Expiration Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Warranty This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
Liability This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.

Immunohistochemistry (IHC):

AE00109 to CD79a was successfully used to stain human tonsil sections. Recommended concentration: 1-3ug/ml

Human tonsil with AE00109
Formaldehyde-fixed, paraffin-embedded human tonsil stained with CD79a Rabbit Recombinant Monoclonal Antibody AE00109 at 1-2ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

Immunocytochemistry (ICC):

AE00109 to CD79a was successfully used to stain vesicles in cell line Raji. Recommended concentration: 1-3ug/ml.

Raji with AE00109
Confocal microscopy of PFA-fixed Raji stained with CD79a Rabbit Recombinant Monoclonal Antibody AE00109 at 1-2ug/ml (1h at ambient temp). Detection by CF488 (green) for the antibody and RedDot (red) for nuclear staining.

Western Blot (WB):

AE00109 to CD79a was successfully used to stain an approx. 50kDa band in lysates of cell line Raji. Recommended concentration: 0.3-1ug/ml

Raji with AE00109
Western Blot of a Raji lysate (30ug) stained with CD79a Rabbit Recombinant Monoclonal Antibody AE00109 at 0.5-1ug/ml (1h at ambient temp). ECL staining by HRP.

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified CD79a Rabbit Recombinant Monoclonal Antibody AE00109. Confirmation of Purity and Integrity of Antibody.

AE00109 Coomassie bands
Integrity of the purified antibody AE00109 under non-reduced and reduced conditions, showing intact IgG at around 130kDa (NR) and intact heavy and light chains at 50kDa and 25kDa resp. (R)

Evidence of mono-specificity:

Specificity and selectivity of AE00109 to CD79a were tested on a human protein array, showing monospecificity, against >19,000 full-length human proteins. A protein BLAST search against H. sapiens reveal the following closely related other protein: CD79B. This related protein was part of the array used and showed no cross-reactivity signals.

AE00109 on HuProt(TM) array
Cross-reactivity assessment of CD79a Rabbit Recombinant Monoclonal Antibody AE00109 (1ug/ml) on CDI's Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

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