PLIN2 Monoclonal Antibody AE00154

520,00

Selective and specific mouse monoclonal antibody clone ADFP/1365 to human PLIN2

See all our PLIN2 antibodies

Official Symbol: PLIN2

GeneID: 123

Swiss-Prot: Q99541

Description

PLIN2

All names and symbols: Adipophilin; Adipose differentiation-related protein; Perilipin-2; ADFP; ADRP

Host speciesMouse
ClonalityMonoclonal
IsotypeIgG1, kappa



Confirmed species reactivityHuman
Confirmed applicationsPA, WB
Aeonian Rating®85
RRIDAB_2747391

Western Blot (WB):

AE00154 to PLIN2 was successfully used to stain an approx. 48kDa band in lysates of cell line Jurkat. Recommended concentration: 1-3ug/ml

WB of Jurkat stained with PLIN2 Mouse Monoclonal Antibody AE00154
RJurkat with AE00154

Western Blot of a Jurkat lysate (30ug) stained with PLIN2 Mouse Monoclonal Antibody AE00154 at 1ug/ml (1h at ambient temp). ECL staining by HRP.



Evidence of mono-specificity (in native PA):

Specificity and selectivity of AE00154 were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed the following closely related protein: PLIN1, 3 and 4. The proteins PLIN1 and 3 were part of the array used and showed no cross-reactivity signals.

AE00154 on protein array
AE00154 on HuProt(TM) array

Cross-reactivity assessment of PLIN2 Mouse Monoclonal Antibody AE00154 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified PLIN2 Mouse Monoclonal Antibody AE00154. Confirmation of Purity and Integrity of Antibody.

Coomassie-stained AE00154
AE00154 Coomassie bands

Integrity of the purified antibody AE00154 under non-reduced and reduced conditions, showing intact IgG at around 130kDa (NR) and intact heavy and light chains at 50kDa and 26kDa resp. (R)

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein G from bioreactor concentrate

Immunogen

Recombinant fragment around aa 249-376 of human PLIN2 protein (exact sequence is proprietary)

Epitope

Within aa 249-376 region

Cellular localization

cytoplasmic lipid droplets, Endoplasmatic Reticulum, ER, plasma membrane

Positive Controls

adrenal gland, cerebellum, HepG2, JAR, liver

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