Calnexin Monoclonal Antibody AE00132

520,00

Selective and specific mouse monoclonal antibody clone CANX/1543 to human CANX

Official Symbol: CANX

GeneID: 821

Swiss-Prot: P27824

Description

Calnexin

All names and symbols: Calnexin; Major histocompatibility complex class I antigen-binding protein p88; CANX; CNX; IP90; P90

Host speciesMouse
ClonalityMonoclonal
IsotypeIgG1, kappa



Confirmed species reactivityHuman
Confirmed applicationsIHC, PA, WB
Aeonian Rating®85
RRIDAB_2736896



Immunohistochemistry (IHC):

AE00132 to Calnexin was successfully used to stain epithelial cells perinuclear in human renal carcinoma sections. Recommended concentration: 1-3ug/ml

IHC of human renal carcinoma stained with CANX Mouse Monoclonal Antibody AE00132
Human renal carcinoma with AE00132

Formaldehyde-fixed, paraffin-embedded human renal carcinoma stained with Calnexin Mouse Monoclonal Antibody AE00132 at 1-2ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

Western Blot (WB):

AE00132 to Calnexin was successfully used to stain an approx. 90kDa band in lysates of cell lines PANC1 and MCF7, and in kidney lysates. Recommended concentration: 1-3ug/ml

WB of PANC1 and MCF7 stained with CANX Mouse Monoclonal Antibody AE00132
PANC1 and MCF7 with AE00132

Western Blot of PANC1 and MCF7 lysates (30ug) stained with Calnexin Mouse Monoclonal Antibody AE00132 at 1-2ug/ml (1h at ambient temp). ECL staining by HRP.

WB of human kidney stained with CANX Mouse Monoclonal Antibody AE00132
Kidney with AE00132

Western Blot of a human kidney lysate (30ug) stained with Calnexin Mouse Monoclonal Antibody AE00132 at 1-2ug/ml (1h at ambient temp). ECL staining by HRP.

Evidence of mono-specificity (in native PA):

Specificity and selectivity of AE00132 to Calnexin were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed the following closely related other protein: CLGN. This protein was part of the array used and showed no cross-reactivity signals.

AE00132 on protein array
AE00132 on HuProt(TM) array

Cross-reactivity assessment of Calnexin Mouse Monoclonal Antibody AE00132 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified Calnexin Mouse Monoclonal Antibody AE00132. Confirmation of Purity and Integrity of Antibody.

Coomassie-stained AE00132
AE00132 Coomassie bands

Integrity of the purified antibody AE00132 under non-reduced and reduced conditions, showing intact IgG at around 140kDa (NR) and intact heavy and light chains at 48kDa and 28kDa resp. (R)

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein G from bioreactor concentrate

Immunogen

Recombinant fragment around aa 1-300 of human Calnexin protein (exact sequence is proprietary)

Epitope

Luminal domain (within aa 1-300 region)

Cellular localization

Endoplasmatic Reticulum, ER

Positive Controls

HeLa, kidney, MCF7, PANC1, small intestine, U2OS

Species reactivity

Human

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