WT1 Recombinant Antibody AE00153

520,00

Specific rabbit recombinant antibody clone WT1/1434R to human WT1

See all our WT1 antibodies

Official Symbol: WT1

GeneID: 7490

Swiss-Prot: P19544

Description

WT1

All names and symbols: Wilms tumor 1; Wilms’ tumor 1; AWT1; GUD; NPHS4; WAGR; WIT-2; WT33

Host speciesRabbit
ClonalityRecombinant
IsotypeIgG
Confirmed species reactivityHuman, Rat
Confirmed applicationsIHC
Aeonian Rating®80
RRIDAB_2744514

Immunohistochemistry (IHC):

AE00153 to WT1 was successfully used to stain nuclei in human mesothelioma and rat testis sections. Recommended concentration: 1-3ug/ml

IHC of human mesothelioma stained with WT1 Rabbit Monoclonal Antibody AE00153
Human mesothelioma with AE00153

Formaldehyde-fixed, paraffin-embedded human mesothelioma stained with WT1 Rabbit Recombinant Antibody AE00153 at 1-2ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

IHC of rat testis stained with WT1 Rabbit Recombinant Monoclonal Antibody AE00153
Rat testis with AE00153

Formaldehyde-fixed, paraffin-embedded rat testis stained with WT1 Rabbit Recombinant Antibody AE00153 at 1-2ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH6 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified WT1 Rabbit Recombinant Antibody AE00153. Confirmation of Purity and Integrity of Antibody.

Coomassie-stained AE00153
AE00153 Coomassie bands

Integrity of the purified antibody AE00153 under non-reduced and reduced conditions, showing intact IgG at around 100kDa (NR) and intact heavy and light chains at 55kDa and 30kDa resp. (R)

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein A from bioreactor concentrate

Immunogen

Recombinant human full-length WT1 protein

Cellular localization

nucleus

Positive Controls

fetal kidney, K562, kidney, mesothelioma, subset of hematopoietic cells, Wilm's Tumor

Epitope

Unknown

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