DNMT3A Monoclonal Antibody AE00167

$520,00

Selective and specific mouse monoclonal antibody clone PCRP-DNMT3A-1E2 to human DNMT3A

Official Symbol: DNMT3A

GeneID: 1788

Swiss-Prot: Q9Y6K1

Description

DNMT3A

All names and symbols: DNA (cytosine-5)-methyltransferase 3A; DNA methyltransferase 3 alpha; DNA methyltransferase HsaIIIA; DNA MTase HsaIIIA; DNMT3A; DNMT3A2; M.HsaIIIA; TBRS

Host speciesMouse
ClonalityMonoclonal
IsotypeIgG1, kappa



Confirmed species reactivityHuman
Confirmed applicationsIHC, PA
Aeonian Rating®80
RRIDAB_2750613



Immunohistochemistry (IHC):

AE00167 to DNMT3A was successfully used to stain nuclei in human placenta sections. Recommended concentration: 1-3ug/ml

IHC of human placenta stained with DNMT3A Mouse Monoclonal Antibody AE00167
Human placenta with AE00167

Formaldehyde-fixed, paraffin-embedded human placenta stained with DNMT3A Mouse Monoclonal Antibody AE00167 at 1-2ug/ml for 30 minutes at RT. Epitope retrieval: Boiling at pH9 for 10-20 min followed by 20 min cooling. DAB staining by HRP polymer.

Evidence of mono-specificity (in native PA):

Specificity and selectivity of AE00167 to DNMT3A were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed the following closely related protein: DNMT3B. This protein was part of the array used and showed no cross-reactivity signals.

AE00167 on protein array
AE00167 on HuProt(TM) array

Cross-reactivity assessment of DNMT3A Mouse Monoclonal Antibody AE00167 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
The Z-score represents the strength of a signal that an antibody (through a fluorophore-tagged secondary reagent) produces when binding to a particular protein on the array. Z-scores are in units of standard deviations (SD’s) above the mean value of all signals generated on that array. When Z-scores are arranged in descending order, the difference between two successive values will be the S-score for the first. Thus, the S-score represents the relative specificity of the antibody to its intended target. An antibody is considered specific to its intended target, when it has an S-score of at least 2.5. For example, if an antibody binds to intended protein X with a Z-score of 43 and to the cross-reacting protein Y with a next Z-score of 14, then the S-score for the antibody to intended target X equals 29 (43-14).

Evidence of molecular integrity:

SDS-PAGE Analysis of Purified DNMT3A Mouse Monoclonal Antibody AE00167. Confirmation of Purity and Integrity of Antibody.

Coomassie-stained AE00167
AE00167 Coomassie bands

Integrity of the purified antibody AE00167 under non-reduced and reduced conditions, showing intact IgG at around 140kDa (NR) and intact heavy and light chains at 50kDa and 23kDa resp. (R)

ExpirationIntegrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
Storage instructionsAvoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
WarrantyThis product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
LiabilityThis product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
Product Data Sheet
Material Safety Data Sheet

Additional information

Purification

By Protein G from bioreactor concentrate

Immunogen

Recombinant full length human DNMT3A protein

Epitope

Unknown

Cellular localization

nucleus

Positive Controls

HeLa, placenta

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